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Nucleic Acids Research, 1989, Vol. 17, No. 22 9305-9318
© 1989

MOLECULAR BIOLOGY

Initiation and termination of human U1 RNA transcription requires the concerted action of multiple flanking elements

Henry E.Neuman de Vegvar+ and James E. Dahlberg*

Department of Physiological Chemistry, University of Wisconsin-Madison Madison, WI 53706, USA

*To whom correspondence should be addressed

Received July 10, 1989. Revised October 11, 1989. Accepted October 11, 1989.

Sequences in the 5' flanking region of small nuclear RNA (snRNA) genes are responsible for recognition of 3' end signals. Formation of the pre-U1 3'end occurs at the downstream signal closest to the promoter, probably by transcription termination. We have analyzed promoter elements for their participation in formation of the 3' ends of pre-U1 RNA. To do this, a human U1 RNA gene with deletions in individual promoter elements was microinjected into Xenopus laevis oocytes and the resulting RNAs were analyzed by a nuclease S1 protection assay. Each of the promoter elements, except element B (the functional equivalent of a TATA box), was shown to be dispensable for recognition of the snRNA 3' end signal. This latter element was necessary, but not sufficient, for initiation of transcription; so its possible role in termination could not be assessed. Therefore, it is likely that recognition of the 3' end signal is an inherent feature of transcription complexes that initiate at an snRNA promoter.

+ Present address: Department of Medicine, Division of Immunology, Stanford University Medical Center, Stanford, CA 94305, USA


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