Skip Navigation

This Article
Right arrow Print PDF (1676K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (26)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Yoshida, K.
Right arrow Articles by Fujinaga, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshida, K.
Right arrow Articles by Fujinaga, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 23 10015-10034
© 1989

MOLECULAR BIOLOGY

Binding sites of HeLa cell nuclear proteins on the upstream region of adenovirus type 5 E1A gene

Koichi Yoshida, Mitsuo Narita and Kei Fujinaga

Department of Molecular Biology, Cancer Research Institute, Sapporo Medical College S-1, W-17, Chuo-ku, Sapporo 060, Japan

Received July 17, 1989. Revised October 27, 1989. Accepted October 27, 1989.

Twenty one binding sites of HeLa cell nuclear proteins were identified on the upstream region of adenovirus type 5 E1A gene using DNase I footprint assay. The proximal promoter region contained five binding sites that overlapped the cap site, TATA box, TATA-like sequence, CCAAT box, and –100 region relative to the E1A cap site(+1). The +190 region was a potential site for octamer-motif binding proteins, such as NFIII and OBP100. An upstream copy of the E1A enhancer element 1 was the site for a factor (E1A-F) with the binding specificity of XGGAYGT(X=A, C; Y=A, T). E1A-F factor also bound to three other sites, one of which coincided with the distal E1A enhancer element. The distal element also contained a potential site for ATF factor. The adenovirus minimal origin of DNA replication competed for DNA-protein complex formation on the CCAAT and TATA box region and the –190 region, suggesting that these regions interacted with a common or related factor.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Biophys. JHome page
C. H. Choi, Z. Rapti, V. Gelev, M. R. Hacker, B. Alexandrov, E. J. Park, J. S. Park, N. Horikoshi, A. Smerzi, K. O. Rasmussen, et al.
Profiling the Thermodynamic Softness of Adenoviral Promoters
Biophys. J., July 15, 2008; 95(2): 597 - 608.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
H. Yamamoto, S. Horiuchi, Y. Adachi, H. Taniguchi, K. Nosho, Y. Min, and K. Imai
Expression of ets-related transcriptional factor E1AF is associated with tumor progression and over-expression of matrilysin in human gastric cancer
Carcinogenesis, March 1, 2004; 25(3): 325 - 332.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
B. Davidson, I. Goldberg, W. H. Gotlieb, J. Kopolovic, G. Ben-Baruch, and R. Reich
PEA3 Is the Second Ets Family Transcription Factor Involved in Tumor Progression in Ovarian Carcinoma
Clin. Cancer Res., April 1, 2003; 9(4): 1412 - 1419.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.