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Nucleic Acids Research, 1989, Vol. 17, No. 23 9637-9648
© 1989

MOLECULAR BIOLOGY

Interaction of a 3' RNA region of the mustard trnK gene with chloroplast proteins

Jörg Nickelsen and Gerhard Link

University of Bochum, Plant Cell Physiology and Molecular Biology D-4630 Bochum, FRG

Received September 7, 1989. Revised November 1, 1989. Accepted November 1, 1989.

The 3' flanking region of the chloroplast trnK gene for tRNALys of mustard contains a palindromic sequence previously implicated with transcription termination and/or processing of the precursor RNA. Here we have investigated whether RNA sequences from the trnK 3' region are capable of interacting with chloroplast proteins in vitro. We find specific binding to an RNA region which is located further downstream from the palindromic sequence. The approximate length and position of this 3' binding region is reflected by a 41 nt spanning RNA segment which is protected against RNase T1 digestion by chloroplast protein(s). Competition experiments and sequence analyses suggest that U residues play an essential role in the RNA-protein interaction. Only a small number of proteins, possibly one single species, is in contact with the trnK 3' RNA.


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C. R. Hauser, N. W. Gillham, and J. E. Boynton
Translational Regulation of Chloroplast Genes
J. Biol. Chem., January 19, 1996; 271(3): 1486 - 1497.
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