Skip Navigation

This Article
Right arrow Print PDF (3189K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (42)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Heidmann, S.
Right arrow Articles by Domdey, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heidmann, S.
Right arrow Articles by Domdey, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 23 9783-9796
© 1989

MOLECULAR BIOLOGY

Cloning, characterization and heterologous expression of the SmaI restriction-modification system

Stefan Heidmann, Wolfgang Seifert, Christoph Kessler1 and Horst Domdey

Laboratorium für Molekulare Biologie, Genzentrum, der Ludwig-Maximilians-Universität München Am Klopferspitz 18a, D-8033 Martinsried 1Boehringer Mannheim GmbH, Biochemical Research Center, Department of Genetics and Molecular Biology Nonnenwald 2, D-8122 Penzberg, FRG

Received August 8, 1989. Revised October 27, 1989. Accepted October 27, 1989.

The genes coding for the class-II Serratia marcescens restnction-modification system have been cloned and expressed in E. coli. Recombinant clones restricted incoming phage only poorly; the recombinant plasmids, however, became fully modified in vivo, i. e. completely resistant against digestion with R SmaI. The determined nucleotide sequence of the cloned system revealed three open reading frames with lengths of 252 bp, 741 bp, and 876 bp. Through various deletion experiments and an insertionmutation experiment the 876 bp open reading flame could be assigned to the SmaI DNA modification enzyme and the 741 bp open reading frame to the SmaI restriction endonuclease. Mapping of the transcription start sites of the genes revealed that the SmaI endonuclease is transcribed as a polycistronic mRNA together with a 252 bp long preceding open reading frame of unknown function. No homology was found when comparing the amino acid sequence of M SmaI with the published sequences of m5C-specific DNA modification methyltransferases. On the other hand, a stretch of 14 amino acids in the C-proximal region of M SmaI shows a significant homology to the C-proximal amino acid sequences of the N6 A-methyltransferases M HinfI and the N4C-methyltransferase M PvuII.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
I. Mruk and R. M. Blumenthal
Real-time kinetics of restriction-modification gene expression after entry into a new host cell
Nucleic Acids Res., May 1, 2008; 36(8): 2581 - 2593.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. Bogdanova, M. Djordjevic, I. Papapanagiotou, T. Heyduk, G. Kneale, and K. Severinov
Transcription regulation of the type II restriction-modification system AhdI
Nucleic Acids Res., March 1, 2008; 36(5): 1429 - 1442.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
I. Mruk and T. Kaczorowski
A Rapid and Efficient Method for Cloning Genes of Type II Restriction-Modification Systems by Use of a Killer Plasmid
Appl. Envir. Microbiol., July 1, 2007; 73(13): 4286 - 4293.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. Semenova, L. Minakhin, E. Bogdanova, M. Nagornykh, A. Vasilov, T. Heyduk, A. Solonin, M. Zakharova, and K. Severinov
Transcription regulation of the EcoRV restriction-modification system
Nucleic Acids Res., December 6, 2005; 33(21): 6942 - 6951.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
D. Knowle, R. E. Lintner, Y. M. Touma, and R. M. Blumenthal
Nature of the Promoter Activated by C.PvuII, an Unusual Regulatory Protein Conserved among Restriction-Modification Systems
J. Bacteriol., January 15, 2005; 187(2): 488 - 497.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
R. M. Vijesurier, L. Carlock, R. M. Blumenthal, and J. C. Dunbar
Role and Mechanism of Action of C {middle dot} PvuII, a Regulatory Protein Conserved among Restriction-Modification Systems
J. Bacteriol., January 15, 2000; 182(2): 477 - 487.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.