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Nucleic Acids Research, 1989, Vol. 17, No. 23 9811-9822
© 1989

MOLECULAR BIOLOGY

Ribosome associated protein(s) specifically bind(s) to the upstream activator sequence of the E. coli rrnA P1 promoter

Nira Nachaliel, Jeffery Melnick, Ron Gafny and Gad Glaser

Department of Cellular Biochemistry, Hadassah Medical School, Hebrew University Jerusalem, Israel

Received August 4, 1989. Revised October 27, 1989. Accepted October 27, 1989.

A sequence located upstream to the E. coli rrnA P1 promoter is required for optimal promoter activity. Deletion of this sequence reduces in vivo transcription by 90%. Substitution of this upstream activating sequence with the unrelated bent DNA sequence of the kinetoplast of Crithidia fasciculata, restores in vivo expression to high levels. Cellular proteins which are present only in exponentially growing cells bind specifically to intact rrnP1, bet do not bind to the promoter missing the upstream asivating sequence. These proteins are associated with the 30S ribosomal subunits but can be washed off with concentrated salt. The correlation between the binding activity and cell growth rate suggests a role for these proteins in the transcriptional control of rRNA synthesis.


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