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Nucleic Acids Research, 1989, Vol. 17, No. 23 9909-9932
© 1989

MOLECULAR BIOLOGY

Cis-acting sequences from mouse rDNA promote plasmid DNA amplification and persistence in mouse cells: implication of HMG-I in their function

Michael Wegner, Gerd Zastrow, Andra Klavinius, Sojna Schwender, Friedemann Müller, Hans Luksza, Jürgen Hoppe1, Johannes Wienberg2 and Friedrich Grummt

Institut für Biochemie, Universität Würzburg D-8700 Würzburg 1Institut für Physiologische Chemie, Universität Würzburg D-8700 Würzburg 2Institut für Anthropologie und Humangenetik Universität München D-8000 München, FRG

Received September 29, 1989. Revised October 30, 1989. Accepted October 30, 1989.

Searching for amplification promoting sequences within the murine rDNA cistrons, we isolated two elements from the nontranscribed spacer region. These 370 bp and 423 bp long cis-acting elements, refered to as muNTS1 and muNTS2, are localized 4.1 kb and 4.6 kb upstream the RNA polymerase I transcriptional start site. They contain Ca. 50 bp long AT-rich sequences that strongly interact with a protein from nuclear extracts. The protein could be purified and identified as HMG-I. A synthetic oligonucleotide encompassing the AT-rich stretch from muNTS1 is able to substitute for the muNTS elements. A similar sequence from the nontranscribed spacer of rat has previously been reported to be important for the function of the RNA polymerase I enhancer (1). Therefore the interaction of HMG I with the muNTS elements may play a role both in the stimulation of DNA amplification and transcription.


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