Nucleic Acids Research, 1989, Vol. 17, No. 3 1035-1050
© 1989
MOLECULAR BIOLOGY |
The 43 residue DNA binding domain of 
resolvase binds adjacent major and minor grooves of DNA
Department of Molecular Biophysics and Biochemistry, Yale University Medical School 333 Cedar Street, PO Box 3333, New Haven, CT 06510, USA
Received October 25, 1988. Accepted January 5, 1989.
The carboxyl-terminal domain of 
resolvase binds to each half of the three resolvase binding sites that constitute the recombination site, res. Ethylation inhibition experiments show that the phosphate contacts made by the C-terminal DNA binding domain are similar to those made by intact resolvase, with the exception of a single phosphate at the inside end of each contact region which is contacted solely by the intact resolvase. The DNA binding domain makes essentially identical contacts to all 6 half sites, whereas the intact resolvase makes slightly different contacts to each binding site. Despite its small size, only 43 amino acid residues, the resolvase C-terminal domain interacts with an unusually large segment of DNA. Phosphate contacts extend across an adjacent major and minor groove of DNA and about one third of the circumference around the helix. The minimal binding segment, determined experimentally, is a 12 bp sequence that includes the 9 base pair inverted repeat (common to all half sites), the adjacent 3 base pairs (towards the center of the intact resolvase binding site), and phosphates at both ends.
+Present address: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
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