Nucleic Acids Research, 1989, Vol. 17, No. 3 1089-1102
© 1989
MOLECULAR BIOLOGY |
Human dopamine ß-hydroxylase gene: two mRNA types having different 3'terminal regions are produced through alternative polyadenylation
Department of Biochemistry, Nagoya University School of Medicine Nagoya 466 1Institute for Comprehensive Medical Science, Fujita-Gakuen Health University Toyoake 470-11, Japan
*To whom all correspondence should be addressed.
Received October 2, 1988. Revised December 20, 1988. Accepted January 9, 1989.
Two kinds of cDNA (types A and B) encoding human dopamine ß-hydroxylase (DBH) were isolated from a pheochromocytoma cDNA library. Type A (2.7 kb) and B (2.4 kb) encoded the same amino acid sequence and were different only in 3'-untranslated region. Type A contained 3'-extension of 300 by at the end of type B. Subsequently, we isolated human DBH gene and analyzed genonic DNA by Southern hybridization. Human DBH gene (approximately 23 kb) was composed of 12 exons and existed as a single gene on genone. Exon 12 encoded 3'-terminal region of 1, 013 by of type A, including the 300 by sequence. These results indicate that alternative use of two polyadenylation sites from a single DBH gene generates different nRNA types. This conclusion was supported by Northern hybridization and S1 nuclease mapping experiments. The ratio of type A and B mRNAs in pheochromocytona was roughly 1.0 to 0.2. We found possible transcription regulatory elements, TATA, CCAAT, CACCC, GC boxes, near the transcription initiation site of DBH gene. Sequences homologous to glucocorticoid and cyclic AMP response elements were also observed.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H. K. Lehman, D. J. Schulz, A. B. Barron, L. Wraight, C. Hardison, S. Whitney, H. Takeuchi, R. K. Paul, and G. E. Robinson Division of labor in the honey bee (Apis mellifera): the role of tyramine {beta}-hydroxylase J. Exp. Biol., July 15, 2006; 209(14): 2774 - 2784. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yamakuni, T. Yamamoto, M. Hoshino, S.-Y. Song, H. Yamamoto, M. Kunikata-Sumitomo, A. Minegishi, M. Kubota, M. Ito, and S. Konishi A Novel Protein Containing cdc10/SWI6 Motifs Regulates Expression of mRNA Encoding Catecholamine Biosynthesizing Enzymes J. Biol. Chem., October 16, 1998; 273(42): 27051 - 27054. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ishiguro, K. Yamada, N. Ichino, and T. Nagatsu Identification and Characterization of a Novel Phorbol Ester-responsive DNA Sequence in the 5'-Flanking Region of the Human Dopamine beta -Hydroxylase Gene J. Biol. Chem., August 21, 1998; 273(34): 21941 - 21949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Houhou, A. Lamouroux, N. F. Biguet, and J. Mallet Expression of Human Dopamine [IMAGE]-Hydroxylase in Mammalian Cells Infected by Recombinant Vaccinia Virus J. Biol. Chem., May 26, 1995; 270(21): 12601 - 12606. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Zullo, J L Kennedy, J Gelernter, M H Polymeropoulos, G Tallini, A J Pakstis, M B Shapiro, C R Merril, and K K Kidd Eliminating mitochondrial DNA competition for nuclear DNA primers. Genome Res., August 1, 1993; 3(1): 39 - 45. [Abstract] [PDF]
|
|
|
|
|
|
A. M. Oyarce, T. C. Steveson, L. Jin, and B. A. Eipper Dopamine beta -Monooxygenase Signal/Anchor Sequence Alters Trafficking of Peptidylglycine alpha -Hydroxylating Monooxygenase J. Biol. Chem., August 24, 2001; 276(35): 33265 - 33272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhou, M. G. Espey, J. X. Chen, L. J. Hofseth, K. M. Miranda, S. P. Hussain, D. A. Wink, and C. C. Harris Inhibitory Effects of Nitric Oxide and Nitrosative Stress on Dopamine-beta -Hydroxylase J. Biol. Chem., July 7, 2000; 275(28): 21241 - 21246. [Abstract] [Full Text] [PDF]
|
|