Nucleic Acids Research, 1989, Vol. 17, No. 3 1121-1138
© 1989
MOLECULAR BIOLOGY |
Identification of the promoter sequences involved in the interleukin-6 dependent expression of the rat 
2-macroglobulin gene
Biochemisches Institut, Universit
t Freiburg D-7800 Freiburg
1Institut für Biochemie Neuklinikum Aachen, Pauwelsstrasse, D-5100 Aachen, FRG
*To whom correspondence should be addressed
Received September 28, 1988. Revised December 20, 1988. Accepted January 13, 1989.
The 5'-region of the rat 
2-macroglobulin gene has been characterized. A 5.6 kb Sal I - Xba I fragment containing the first 4 exons of the 2-macroglobulin gene and 1.3 kb of its 5'-flanking region was sequenced. The putative transcriptional start site was determined by RNase protection and primer extension analysis. TATA- and CAAT-box equivalent sequences were found. A potential glucocorticoid receptor binding site was located on the antisense strand.
DNA sequences containing the 5'-flanking region of the rat 2-macroglobulin gene were linked to the gene coding for the bacterial cloramphenicol acetyltransferase and introduced into Hep G2 cells. In these transfected Hep G2 cells CAT activity could be induced by recombinant human interleukin-6. Deletion analyses have shown that the sequences between –852 and –777 as well as between –404 and –165 relative to the cap site, contain regulatory elements involved in the interleukin-6 dependent induction of the 2-macroglobulin gene.
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