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Nucleic Acids Research, 1989, Vol. 17, No. 3 1139-1157
© 1989

MOLECULAR BIOLOGY

Terminal sequences do not contain the rate-limiting decay determinants of E.coli cat mRNA

Christopher DeFranco and Janet L. Schottel

Department of Biochemistry, University of Minnesota 1479 Gortner Avenue, St Paul, MN 55108, USA

Received September 1, 1988. Revised December 27, 1988. Accepted December 27, 1988.

The mechanism of E. coli chloramphenicol acetyltransferase (cat) mRNA decay was investigated. Alteration of the 5' untranslated terminus does not appear to have an effect on the turnover rate of the mRNA. Similarly, changes at the 3' terminus of the message, including the addition of a stable stem and loop structure, do not affect the half-life of the message. The data suggest that 5' and 3' terminal untranslated sequences do not contain the rate-limiting determinants for cat message decay. Decay rates for various segments of the cat mRNA were measured and indicate that all regions of the message have similar stabilities. The current model of cat mRNA degradation involves a rate-limiting endonucleolytic decay event that occurs internal to the message followed by degradation of the cleavage products.


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