Skip Navigation

This Article
Right arrow Print PDF (5644K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (28)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Karaimann, G.
Right arrow Articles by Högenauer, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Karaimann, G.
Right arrow Articles by Högenauer, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 4 1283-1298
© 1989

MOLECULAR BIOLOGY

A stable core region of the tra operon mRNA of plasmid R1-19

Günther Karaimann and Gregor Högenauer*

Institut für Mikrobiologie, Karl-Franzens-Universität Graz A-8010 Graz, Austria

*To whom correspondence should be addressed

Received January 5, 1989. Accepted January 23, 1989.

The degradation of the polycistronic tra-mRNA of the resistance plasmid R1-19 leads to the accumulation of a well defined series of stable mRNA species. The majority of the most stable mRNAs contains the message for the traA gene only. The differently sized stable mRNAs possess a common 3'terminus within the traL gene but vary at their 5'ends. The 3'terminus probably results from protection against exoribonucleases by a secondary structural feature. We propose that the 5'ends are generated by endoribonucleolytic cleavage. The stability of this part of the tra-mRNA exceeds 30 minutes and probably increases the rate of expression of the traA gene product propilin, the precursor of the sex pilus subunit. The expression of propilin and its processing into a protein of the molecular weight of mature pilin is demonstrated with the isolated gene. The sequence of the so far unknown genes traL and traE of R1-19 is presented.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
X. Yang, Q. Ma, and T. K. Wood
The R1 Conjugative Plasmid Increases Escherichia coli Biofilm Formation through an Envelope Stress Response
Appl. Envir. Microbiol., May 1, 2008; 74(9): 2690 - 2699.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
D. Zahrl, A. Wagner, M. Tscherner, and G. Koraimann
GroEL Plays a Central Role in Stress-Induced Negative Regulation of Bacterial Conjugation by Promoting Proteolytic Degradation of the Activator Protein TraJ
J. Bacteriol., August 15, 2007; 189(16): 5885 - 5894.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
W. R. Will and L. S. Frost
Characterization of the Opposing Roles of H-NS and TraJ in Transcriptional Regulation of the F-Plasmid tra Operon
J. Bacteriol., January 15, 2006; 188(2): 507 - 514.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. J. Gubbins, I. Lau, W. R. Will, J. M. Manchak, T. L. Raivio, and L. S. Frost
The Positive Regulator, TraJ, of the Escherichia coli F Plasmid Is Unstable in a cpxA* Background
J. Bacteriol., October 15, 2002; 184(20): 5781 - 5788.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. G. Anthony, W. A. Klimke, J. Manchak, and L. S. Frost
Comparison of Proteins Involved in Pilus Synthesis and Mating Pair Stabilization from the Related Plasmids F and R100-1: Insights into the Mechanism of Conjugation
J. Bacteriol., September 1, 1999; 181(17): 5149 - 5159.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.