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Nucleic Acids Research, 1989, Vol. 17, No. 4 1315-1326
© 1989

MOLECULAR BIOLOGY

Pulse-field linkage of the P3, G6pd and Cf-8 genes on the mouse X chromosome: demonstration of synteny at the physical level

N. Brockdorff, L. C. Amar1 and S. D. M. Brown2,*

Clinical Research Centre, Comparative Biology Section Watford Road, Harrow, Middlesex HA1 3UJ, UK 1Unite d'Immunogenetique Humaine, INSERM U276, Institut Pasteur 25 rue de Docteur Roux, 75724 Paris Cedex 15, France 2Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School Norfolk Place, London W2 1PG, UK

*To whom correspondence should be addressed

Received November 23, 1988. Accepted January 26, 1989.

Utilising pulse-field gel electrophoresis physical linkage between three mouse X-linked genes has been demonstrated. The three genes, P3, G6pd and Cf-8 all lie within 400Kb of DNA. This physical linkage mirrors the situation on the human X chromosome, respresenting the first demonstration of mouse/human synteny at the physical level. A detailed physical map encompassing 1.6 Mbp of this region is presented. A number of the rare cutter restriction enzyme sites within this map are partially blocked on the inactive X chromosome, presumably due to the methylation of CpG rich islands. Pulsed field gel electrophoresis therefore provides a useful tool for the study of X-inactivation over large regions of the X chromosome.


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