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Nucleic Acids Research, 1989, Vol. 17, No. 4 1371-1377
© 1989

MOLECULAR BIOLOGY

A catalytic 13-mer ribozyme

Alex C. Jeffries and Robert H. Symons*

Department of Biochemistry, University of Adelaide Adelaide, SA 5000, Australia

*To whom correspondence should be addressed

Received January 3, 1989. Accepted January 26, 1989.

A 13-mer oligoribonucleotide can act as a ribozyme for the specific self-cleavage of a 41-mer oligoribonucleotide substrate in the presence of Mg2+ The two sequences involved correspond to the self-cleavage hammerhead structure of the virusoid of lucerne transient streak virus. The Michaelis-Menten kinetic parameters for the reaction were; Km 1.3 µM, Vmax 0.012 µM min–1 kcat 0.5 mm–1. The 13-mer RNA is the smallest ribozyme so far reported. A DNA analogue of the 13-mer can not substitute for the RNA in the reaction.


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