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Nucleic Acids Research, 1989, Vol. 17, No. 4 1563-1572
© 1989

MOLECULAR BIOLOGY

Protein-DNA conjugates produced by UV irradiation and their use as probes for hybridization

Joachim Czichos, Martin Köhler, Bernd Reckmann1 and Manfred Renz

Medizinisch-Technisches Laboratorium Denagen, Im Neuenheimer Feld 517, D-6900 Heidelberg 1E.Merck, D-6100 Darmstadt, FRG

Received November 4, 1988. Accepted January 19, 1989.

Several proteins have been crosslinked to DNA by low dose uv irradiation. The principle of the method is based on an efficient and fast radiation induced reaction of amino acid residues with DNA at low pH. The method seems to be of general applicability for crosslinking proteins to DNA in a very simple one step procedure. Some of such DNA-protein conjugates have been used as probes for hybridization experiments. DNA-protein A probes were found to be most useful.


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