Skip Navigation

This Article
Right arrow Print PDF (4871K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Pruijn, G. J.M.
Right arrow Articles by Mattaj, I. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pruijn, G. J.M.
Right arrow Articles by Mattaj, I. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 5 1845-1863
© 1989

MOLECULAR BIOLOGY

Anti-OTF-1 antibodies inhibit NFIII stimulation of in vitro adenovirus DNA replication

Ger J.M. Pruijn*, Peter C. van der Vliet, Nina A. Dathan1 and Iain W. Mattaj1

Laboratory for Physiological Chemistry, State University of Utrecht Vondellaan 24a, 3521 GG Utrecht, The Netherlands 1European Molecular Biiology Laboratory Meyerhofstrasse 1, 6900 Heidelberg, FRG

Received January 18, 1989. Revised February 13, 1989.

HeLa cell OTF-1 has been purified on the basis of its DNA binding activity and used to raise a polyclonal rabbit antiserum. This antiserum is shown to recognize both native and denatured OTF-1 from both human and a similar protein from Xenopus culture cells, but to react either more weakly or not at all with the lyniphoid cell-specific OTF-2. Separately, NFIII has been purified on the basis of its ability to stimulate Adenovirus DNA replication in vitro. On denaturing polyacrylamide gels OTF-1 and NFIII exhibit identical mobility. Anti-OTF-1 antiserum recognizes NFIII and neutralizes its stimulatory effect on DNA replication. Moreover, OTF-1 can functionally replace NFIII. Taken together with previously published DNA binding data, this indicates that OTF-1 and NFIII are either very closely related or identical.

*Present address: Department of Biochemistry, University of Nijmegen, Trigon, PO Box 9101, 6500 HB Nijmegen, The Netherlands


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.