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Nucleic Acids Research, 1989, Vol. 17, No. 5 1977-1987
© 1989

MOLECULAR BIOLOGY

Regulatory elements involved in the bidirectional activity of an immunoglobulin promoter

Noëlle Doyen, Marc Dreyfus and François Rougeon

Unité de Génétique et Biochimie du Développement, LA CNRS 361, Departement d'Immunologie, Institut Pasteur 25 rue du Dr Roux, 75724 Paris Cedex 15, France

Received October 25, 1988. Revised February 9, 1989. Accepted February 9, 1989.

We show that the promoter from the mouse VH441 heavy-chain immunoglobulin gene, when present on plasmids transiently introduced into myeloma cells, promotes transcription bidirectionally, due to the presence on both strands of TATA-like sequences bracketting the highly conserved decanucleotide element. The two divergent promoters compete for the transcriptional machinery, their relative strength ultimately reflecting the likeness of the two TATA boxes to the consensus sequence. Moreover, their relative activity is also strongly influenced by certain point mutations within the distally located heavy-chain enhancer. The bearing of these results on current concepts of promoter function is discussed.


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