Skip Navigation

This Article
Right arrow Print PDF (1801K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hauer, C. A.
Right arrow Articles by Tykocinski, M. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hauer, C. A.
Right arrow Articles by Tykocinski, M. L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 5 1989-2003
© 1989

MOLECULAR BIOLOGY

Epstein - Barr virus episome-based promoter function in human myeloid cells

Christopher A. Hauer+, Robert R. Getty and Mark L. Tykocinski*

Institute of Pathology, Case Western Reserve University Cleveland, OH 44106, USA

*To whom correspondence should be addressed

Received October 21, 1988. Revised February 7, 1989. Accepted February 7, 1989.

Epstein-Barr virus (EBV) episomal replicons offer an expeditious means for amplifying transfected genes in human cells. A panel of EBV episomes was constructed to assess the relative utility of five distinct eukaryotic promoter elements for high level and inducible gene expression in stably transfected human myeloid leukemia cells. The Rous sarcoma virus 3' long terminal repeat (LTR) was most highly suited for EBV episome-based gene expression, whereas the lymphopapilloma virus and the SV40 early regulatory elements exhibited substantially lower activities. Chemically responsive promoter elements, such as the SV40 early, human metallothionein II and rat GRP78 gene promoters, retained their inducibility when ÊBV episome-based. Differences in gene expression obtained with the episomes reflected differential promoter activity rather than significant variations in episome copy numbers per cell. These observations provide guidelines for the optimal design of EBV episomal expression vectors for human expression work.

+Present address: Department of Pathology, Cleveland Metropolitan General Hospital, Cleveland, OH 44106, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
B. T. Tan, L. Wu, and A. J. Berk
An Adenovirus-Epstein-Barr Virus Hybrid Vector That Stably Transforms Cultured Cells with High Efficiency
J. Virol., September 1, 1999; 73(9): 7582 - 7589.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
V. V. Bartsevich and H. B. Pakrasi
Membrane Topology of MntB, the Transmembrane Protein Component of an ABC Transporter System for Manganese in the Cyanobacterium Synechocystis sp. Strain PCC 6803
J. Bacteriol., June 1, 1999; 181(11): 3591 - 3593.
[Abstract] [Full Text]

Home page
 BloodHome page
T. H. Howard, J. Hartwig, and C. Cunningham
Lymphocyte-Specific Protein 1 Expression in Eukaryotic Cells Reproduces the Morphologic and Motile Abnormality of NAD 47/89 Neutrophils
Blood, June 15, 1998; 91(12): 4786 - 4795.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
P. D'Aloja, E. Olivetta, R. Bona, F. Nappi, D. Pedacchia, K. Pugliese, G. Ferrari, P. Verani, and M. Federico
gag, vif, and nef Genes Contribute to the Homologous Viral Interference Induced by a Nonproducer Human Immunodeficiency Virus Type 1 (HIV-1) Variant: Identification of Novel HIV-1-Inhibiting Viral Protein Mutants
J. Virol., May 1, 1998; 72(5): 4308 - 4319.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
K.-F. Lin, J. Chao, and L. Chao
Human Atrial Natriuretic Peptide Gene Delivery Reduces Blood Pressure in Hypertensive Rats
Hypertension, December 1, 1995; 26(6): 847 - 853.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.