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Nucleic Acids Research, 1989, Vol. 17, No. 6 2197-2201
© 1989

ENZYMOLOGY

Polymerase chain reaction: replication errors and reliability of gene diagnosis

Michael Krawczak, Jochen Reiss*, Jörg Schmidtke and Uwe Rösler1

Institut für Humangenetik Gosslerstrasse 12 d, D-3400 Göttingen, FRG 1institut für Mathematische Stochastik Lotzestrasse 13, D-3400 Gottingen, FRG

*To whom correspondence should be addressed

Received January 6, 1989. Revised February 16, 1989. Accepted February 16, 1989.

The impact of replication errors on the reliability of polymerase chain reaction (PCR) data is studied theoretically. Practical applications of our results to RFLP analysis and oligonucleotide probing confirm that for practical purposes replication errors can be neglected if a large number of starting templates (e.g. 100,000) is being used. For single locus analysis in single cells, however, the probability of false diagnosis due to such errors is of the order of 1 percent.


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