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Nucleic Acids Research, 1989, Vol. 17, No. 7 2379-2390
© 1989

CHEMISTRY

Solid-phase synthesis of oligoribonucleotides using 9-fluorenylmethorycarbunyl (Fmoc) for 5'-hydroryl protection

Christian Lehman+, Yao-Zhong Xu0, Chris Christodoulou§, Zu-kun Tanø and Michael J. Gait*

Medical Research Council, Laboratory of Molecular Biology Hills Road, Cambridge CB2 2QH, UK +Laboratorium fur Organische Chemie, ETH-Zenturum, Universitatstrasse 16, Ch-8092, Zurich, Switzerland 0Shanghai Institute of Organic Chemistry Academia Sinica, 345 Lingling Road, Shanghai, China §Department of Genetics Glaxo Group Research Greenford Road, Greenford, Middlesex UB6 0HE, UK øInstitute of Biophysics, Academia Sinica, Peking 345 Lingling Road, shanghai, china

*To whom correspondence should be addressed

Received February 9, 1989. Accepted March 3, 1989.

Efficient solid-phase synthesis of a series of oligoribonucleotides of up to 20 residues is described that utilises the 9-fluorenylmethoxycarbonyl group (Fmoc) for 5'-protection and 4-methoxytetrahydropyran4-yl (Mthp) for 2'-protection of ribonucleotide monomers and a phosphorarnidite coupling procedure. The Fmoc group is removed after each coupling step by treatment with 0.1M DBU in acetonitrile. Oligoribonucleotides are isolated in 2'-protected form in good yield and shown to be readily and efficiently deprotected by mild acidic treatment.


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