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Nucleic Acids Research, 1989, Vol. 17, No. 7 2493-2502
© 1989


MOLECULAR BIOLOGY

Kinetics of exchangeable protons in Z DNA: a UV reaonance Raman study

Alain Laigle, Laurent Chinsky, Pierre-Yves Turpin and Béatrice Jollès

LPCB, CNRS, UA 198, Institut Curie, 11 rue P. et M. Curie, 75231 Paris Cedex 05, France

Received October 18, 1988. Revised February 28, 1989. Accepted February 28, 1989.

We have studied the hydrogen-deuterium exchange kinetics of the exchangeable protons of the poly(dG-dC).poly(dG-dC) in the Z form of the polymer, using resonance Raman spectroscopy with 257 nm and 284 nm excitation wavelengths.

In our experimental conditions (4.5 M NaCl, phosphate buffer pH7, 2°C) the two amino protons and the imino proton of guanine are exchanged with the same exchange half-time of 13 min, whereas the two amino protons of cytosine are exchanged with the same exchange half-time of 51 min.


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