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Nucleic Acids Research, 1989, Vol. 17, No. 7 2529-2540
© 1989

MOLECULAR BIOLOGY

Occurrence and functional compatibility within Enterobacteriaceae of a tRNA species which inserts selenocysteine into protein

Johann Heider, Walfred Leinfelder and August Böck*

Lehrstuhl fur Mikrobiologie der Universitat Munchen, Maria-Ward-Straßle la, D-8000 München 19, FRG

*To whom correspondence should be addressed

Received February 14, 1989. Accepted March 3, 1989.

The selC gene from E. coli codes for a tRNA species (Formula)which is aminoacylated with L-serine and which co-translationally inserts seleriocysteine into selenoproteins. By means of Southern hybridization it was demonstrated that this gene occurs in all enterobacteria tested. To assess whether the unique primary and secondary structural features of the E. coli selC gene product are conserved in that of other organisms, the selC homologue from Proteus vulgaris was cloned and sequenced. It was found that the Proteus selC gene differs from the E. coli counterpart in only six nucleotides, that it displays the same unique properties and that it is expressed and functions in E. coli. This indicates that the unique mechanism of selenocysteine incorporation is not restricted to E. coil but has been conserved as a uniform biochemical process.


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