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Nucleic Acids Research, 1989, Vol. 17, No. 7 2613-2622
© 1989

MOLECULAR BIOLOGY

Three mitochondrial tRNA genes from Arabidopsis thaliana: evidence for the conversion of a tRNAPhe gene into a tRNATyr gene

Hsu-Ching Chen1,2,ø, Henri Wintz1,+, Jacques-Henry Weil2 and Datta T.N. Pillay1,*

1Department of Biological Sciences, University of Windsor Windsor, Ontario N9B 3P4, Canada 2Institut de Biologie Moléculaire des Plantes 12 rue du Général Zimmer, 67000 Strasbourg, France

To whom correspondence should be addressed

Received January 20, 1989. Accepted February 27, 1989.

Three tRNA genes have been isolated from a genomic library of Arabidopsis thaliana: a tRNASer (GCU), a tRNATyr (GUA) and a tRNAGlu (UUC) genes. These genes are located closely on the same DNA fragment. The tRNASer and the tRNAGlu genes have both 99% sequence similarity with their mitochondrial counterparts from higher plants indicating that these three tRNA genes are mitochondrial. The tRNATyr gene shows a particular high sequence similarity with the mitochondrial tRNAphe pseudogene from maize, and both genes are flanked by a tRNASer gene in the upstream region. Extensive sequence comparisons of the Arabidopsis thaliana mitochondrial sequence containing the three tRNA genes and the corresponding region from maize and soybean mitochondria have shown evidence that the TRNATyr gene has been generated from a mitochondrial tRNAphe gene. The conversion was accomplished by three genetic events: a 4 base-pair deletion, a mutation and a recombination, which led to the transformation of the acceptor stem and the anticodon.

+Present addresses: Department of Genetics and Development, Cornell University, Ithaca, NY 14853

øPresent addresses: Boyce Thompson Institute, Cornell University, Tower Road, Ithaca, NY 14853, USA


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