Skip Navigation

This Article
Right arrow Print PDF (452K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (38)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Paoletti, J.
Right arrow Articles by Paoletti, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Paoletti, J.
Right arrow Articles by Paoletti, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 7 2693-2704
© 1989

CHEMISTRY

{alpha}-DNA VIII: thermodynamic parameters of complexes formed between the oligo-alpha-deoxynucleotides: {alpha}-d(GGAAGG) and {alpha}-d(CCTTCC) and their complementary oligo-beta-deoxynucleotides: ß-d(CCTTCC) and ß-d(GGAAGG) are different

Jacques Paoletti, Didier Bazile, François Morvan1, Jean-Louis Imbach1 and Claude Paoletti

Unité de Biochemie, LA 147 CNRS and U 140 INSERM, Institut Gustave Roussy, 94800 Villejuif 1Laboratoire de Chimie Bio-organique, UA 488 CNRS, Université des Sciences et Techniques du Languedoc, 34060 Montpellier Cédex, France

Received December 2, 1988. Revised February 21, 1989. Accepted March 6, 1989.

The temperature dependence of the formation of a complex between an {alpha}-d(CCTTCC) hexanucleotide and its complementary ß-d(GGAAGG) sequence was studied and compared to the formation of the ß-d(CCTTCC):ß-d(GGAAGG) complex. Such {alpha}-ß complex is more stable than the regular ß:ß complex. The Tm value for the {alpha}:ß complex is 28°C ({Delta}G° = –7.3 kcal/mole) while Tm=20,1°C ({Delta}G° = –6.3 kcal/mole) for the ß:ß complex. The stoechiometry of the {alpha}:ß complex corresponds to the formation of a 1:1 duplex.

However, when the {alpha}- strand is made of {alpha}-purines: {alpha}-d(GGAAGG), the stability of the {alpha}:ß complex, {alpha}-d(GGAAGG): ß-d(CCTTCC) is found to be lower (Tm = 13.8°C) than the stability of the regular ß-ß complex, leading to the conclusion that the nature of the {alpha}-sequence is important in terms of stability when considering the synthesis of such a sequence for using it as antisense oligonucleotide.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.