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Nucleic Acids Research, 1989, Vol. 17, No. 7 2769-2782
© 1989

MOLECULAR BIOLOGY

Definition of a second dimeric subfamily of human {alpha} satellite DNA

James D. Thompson, James E. Sylvester1,*, Iris Laudien Gonzalez1,*, Carl C. Costanzi and David Gillespie

Department of Neoplastic Diseases, Hahnemann University Philadelphia, PA 19102 1Department of Human Genetics, University of Pennsylvania Philadelphia, PA 19104, USA *Present address: Department of Pathology, Hahnemann University Philadelphia, PA 19102, USA

Received November 16, 1988. Revised February 27, 1989. Accepted February 27, 1989.

We describe a new human subfamily of alpha satellite DNA. The restriction endonuclease XbaI cleaves this subfamily into a collection of fragments which are heterogeneous with respect to size. We compared the sequences of 6 clones from four different XbaI size classes. Clones from a single size class were not necessarily more related than clones from different classes. Clones from different size classes were found to produce almost identical hybridization patterns with XbaI-digested human genomic DNA. All clones were found to share a common dimeric repeat organization, with dimers exhibiting about 84% sequence identities, indicating that the clones evolved from a common progenitor alphoid dimer. We show that that this subfamily, and the EcoRI dimer subfamily originally described by Wu and Manuelidis (1), evolved from different progenitor alphoid dimers, and therefore represent distinct human alphoid subfamilies.


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