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Nucleic Acids Research, 1989, Vol. 17, No. 8 3023-3036
© 1989

ENZYMOLOGY

Unusual promoter-independent transcription reactions with bacteriophage RNA polymerases

Guido Krupp

Institute for General Microbiology Olshausenstrasse 40, D-2300 Kiel, FRG

Received January 17, 1989. Revised March 21, 1989. Accepted March 21, 1989.

Efficient transcription reactions of DNA-dependent RNA polymerases require the presence of a specific promoter sequence. This report shows that in the absence of their cognate promoter, two bacteriophage RNA polymerases are capable of performing unusual transcription reactions: (i) the DNA template serves also as a primer for RNA synthesis and this leads to hybrid DNA/RNA molecules, (ii) if the DNA template forms a hairpin structure, the linear DNA can be transcribed via the ‘rolling circle’ mechanism.


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