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Nucleic Acids Research, 1989, Vol. 17, No. 8 3117-3128
© 1989

MOLECULAR BIOLOGY

Differential extra-renal expression of the mouse renin genes

Christopher C.J. Miller*, Andrew T. Carter, Jeanie I. Brooks, Robin H. Lovell-Badge1 and William J. Brammar

Leicester University/ICI Joint Laboratory, Department of Biochemistry, University of Leicester University Road, Leicester LE1 7RH, UK 1MRC Mammalian Development Unit Wolfson House, 4 Stephenson Way, London NW1 2HE, UK

*To whom correspondence should be addressed

Received January 31, 1989. Revised March 16, 1989. Accepted March 16, 1989.

We have used RNase-protection analyses to study renin gene expression in one- and two-gene mouse strains. The RNase-protection assay is capable of discriminating between the transcripts from the different renin genes. In a two-gene strain containing Ren-1D and Ren-2, we demonstrate transcriptional activity from Ren-1D in kidney, submandibular gland (SMG), testes, liver, brain and heart. Ren-2 is clearly expressed in kidney, SMG and testes. Similar analyses of one gene strains (containing Ren-1C only) show expression in kidney, SMG, testes, brain and heart. We cannot detect renin mRNA in the liver of these mice. Ren-1C and Ren-1D thus display quite different tissue-specificities. In order to determine whether the different tissue-specificities of the highly homologous Ren-1C and Ren-1D genes are due to different trans-acting factors in the different mouse strains or to different cis-acting DNA elements inherent to the genes, we introduced a Ren-1D transgene (Ren-1*) into a background strain containing only the Ren-1C gene. The transgene exhibits the same tissue-specificity as the Ren-1D gene of two-gene strains suggesting the presence of different cis-acting DNA elements in Ren-1C and Ren-1D.


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