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Nucleic Acids Research, 1990, Vol. 18, No. 1 111-117
© 1990

MOLECULAR BIOLOGY

A functional splice site in the 5' untranslated region of a zein gene

John W.S. Brown* and Gunter Feix

Institut für Biologie III, Albert Ludwigs Universität D-7800 Freiburg, FRG

Received September 22, 1989. Accepted December 1, 1989.

Zein genes, the genes coding for the zein storage proteins of maize, have a unique gene structure where at least two promoters lie upstream of the coding region. Between the P1 promoter (900 base pairs upstream of the coding region) and the translation initiation AUG codon are 18 short reading frames. A discrepancy between the signals obtained by S1-mapping and primer extension and the DNA sequence in the region of one of these signals suggests the presence of a 3' splice site lying 40 nucleotides upstream of the coding region. A splicing event removing all of the short reading frames from the mRNA transcribed from the P1 promoter would bring this mRNA into a translatable form. Further evidence for a functional 3' splice site has been obtained from sequencing of primer extension products and in vitro splicing of a hybrid intron in the HeLa cell in vitro splicing system.

* Present address: Department of Biological Sciences, University of Dundee, Dundee DD1 4HN, UK


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