Skip Navigation

This Article
Right arrow Print PDF (6460K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (82)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Groot, R. P.d.
Right arrow Articles by Kruijer, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Groot, R. P.d.
Right arrow Articles by Kruijer, W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1990, Vol. 18, No. 11 3195-3202
© 1990

MOLECULAR BIOLOGY

Differential expression ofjun and fosgenes during differentiation of mouse P19 embryonal carcinoma cells

Rolf P.de Groot, Jon Schoorlemmer, Siebe T.van Ganesen and Wiebe Kruijer*

Hubrecht Laboratory, Netherlands Institute for Development Biology, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands

To whom correspondents should be addressed

Received March 14, 1990. Accepted May 11, 1990.

The jun and fos gene families encode DNA binding proteins involved in transcrlptional regulation of genes containing a TPA responsive element (TRE). To study their role in gene regulation during early mammalian development, expression and transcription regulatory properties of their gene products were investigated during retinoic acid (RA) induced differentiation of P19 embryonal carcinoma (EC) cells. Our results show, that c-jun is expressed at low but detectable levels in undifferentlated P19 EC cells and at elevated levels in its RA differentiated derivatives, corresponding with increased expression of Jun and TRE binding activity. Jun D is constitutively expressed at constant levels in both undifferentlated and differentiated P19 cells, while jun B and c-fos are not expressed. Addition of TPA to undifferentiated P19 cells does not result in induction of c-jun, jun B and c-fos, while these genes are transiently induced in RA-differentiated P19 cells. In addition, TPA treatment resulted in expression of Fos and Jun protein in RA-differentiated, but not in undifferentiated P19 cells. Addition of TPA to P19 EC cells expressing low levels of TRE binding proteins is neither followed by transcriptional activation of the TRE reporter gene nor by induction of c-jun, previously shown to be autoregulated by its own gene product. By contrast, in P19 cells differentiated by RA that contain elevated levels of TRE binding proteins, TRE dependent transcription is enhanced upon TPA treatment.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
C. Jin, H. Li, T. Murata, K. Sun, M. Horikoshi, R. Chiu, and K. K. Yokoyama
JDP2, a Repressor of AP-1, Recruits a Histone Deacetylase 3 Complex To Inhibit the Retinoic Acid-Induced Differentiation of F9 Cells
Mol. Cell. Biol., July 1, 2002; 22(13): 4815 - 4826.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. H. G. Verheijen, R. M. F. Wolthuis, J. L. Bos, and L. H. K. Defize
The Ras/Erk Pathway Induces Primitive Endoderm but Prevents Parietal Endoderm Differentiation of F9 Embryonal Carcinoma Cells
J. Biol. Chem., January 15, 1999; 274(3): 1487 - 1494.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.