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Nucleic Acids Research, 1990, Vol. 18, No. 11 3219-3225
© 1990

MOLECULAR BIOLOGY

Characterrisation of sequences required for RNA initiation from the PGK promoter of Saccharomyces cerevisiae

J. Rathejen and J. Mellor*

Department of Biochemistry, University of Oxford South Parks Road, Oxord OX1 3QU, UK

To whom correspondents should be addressed

Received March 9, 1990. Accepted May 11, 1990.

A single copy Dictyostelium gene was dissected and elements responsible for its complex pattern of regulation were defined by transcript analysis of gene fusions. Two overlapping promoters responsible for the transcription of an ‘L’ and an ‘S’ mRNA could be defined. Further dissection of the P8A7 L promoter resulted in the identification of a sequence necessary for stress induction and an element required for vegetative expression. The P8A7 S promoter could be reduced to 449 bp which were sufficient for expression in developing cells. The sequence element required for this transcriptional activity was shown to reside in a 51 bp fragment. Our results show that differential expression of the P8A7 gene is mediated by two independently functioning promoters which, however, share some regulatory elements. A third nuclear RNA species ‘P’ was due to the stress-sensitivity of the 3' processing signal.


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