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Nucleic Acids Research, 1990, Vol. 18, No. 12 3495-3502
© 1990

MOLECULAR BIOLOGY

Poly(dA) • poly(dT) rich sequences are not sufficient to exclude nucleosome formation in a constitutive yeast promoter

Riccardo Losa+, Stefano Omari and Fritz Thoma*

Institut für Zellbiologie, ETH-Hönggerberg CH-8093 Zürich, Switzerland

*To whom correspondence should be addressed

Received March 28, 1990. Revised May 10, 1990. Accepted May 10, 1990.

It was suggested that poly(dA) • poly(dT) rich sequences in yeast Saccharomyces cerevisiae act as elements of constitutive promoters by exclusion of nucleosomes (Struhl, K. (1985). Proc. Natl. Acad. Sci. USA 82, 8419–8423). We have mapped the chromatin structure of the pet56-his3-ded1 region in minichromosomes and show that the poly(dA) • poly(dT) sequences are located in nuclease sensitive regions. DNA fragments from the nuclease sensitive promoter region of DED1 were used for nucleosome reconstitution in vitro. We show that all sequences can form nucleosome cores and that the poly(dA) • poly(dT) sequence can be incorporated in nucleosome cores. The results suggest that the nuclease sensitivity found in vivo is not established by poly(dA)•poly(dT) mediated exclusion of nucleosomes.

+Present address: F.Marti AG, CH-9001 St Gallen, Switzerland


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