Skip Navigation

This Article
Right arrow Print PDF (2305K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Barlett, M. M.
Right arrow Articles by Meyer, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barlett, M. M.
Right arrow Articles by Meyer, R. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1990, Vol. 18, No. 12 3579-3586
© 1990

MOLECULAR BIOLOGY

Recombination between directly repeated origins of conjugative transfer cloned in M13 bacteriophage DNA models ligation of the transferred plasmid strand

Marilyn M. Barlett, Matthew J. Erickson and Richard J. Meyer*

Department of Microbiology, ESB 226, University of Texas at Austin Austin, TX 78712, USA

*To whom correspondence should be addressed

Received February 28, 1990. Revised May 16, 1990. Accepted May 16, 1990.

When two, directly-repeated copies of the origin of transfer (oriT) of the conjugatively mobilizable, broad host-range plasmid R1162 are cloned into bacteriophage M13mp9 DNA, they undergo recombination in the presence of one of the R1162-encoded proteins required for mobilization [Meyer, R. (1989) J. Bacteriol., 171, 799–806]. Mutations in the outer arm of the inverted repeat within oriT inhibit this recombination. These mutations also affect a late step in transfer. We propose that recombination on the phage DNA models the processing of single-stranded DNA after entry into a recipient cell. The two, directly-repeated oriTs are not equivalent during the recombination reaction, because they are differently affected by the outer-arm mutations. A mutation was also isolated that reduces the specificity of the cleavage site in one of the two oriTs. Together, the results with the mutations suggest that phage recombinants can form only when the first cleavage occurs at one of the two oriTs. This is followed by the resulting free 3' end joining to the 5' end at the cleavage site of the other oriT.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Bacteriol.Home page
S. Jandle and R. Meyer
Stringent and Relaxed Recognition of oriT by Related Systems for Plasmid Mobilization: Implications for Horizontal Gene Transfer
J. Bacteriol., January 15, 2006; 188(2): 499 - 506.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
N. Furuya and T. Komano
NikAB- or NikB-Dependent Intracellular Recombination between Tandemly Repeated oriT Sequences of Plasmid R64 in Plasmid or Single-Stranded Phage Vectors
J. Bacteriol., July 1, 2003; 185(13): 3871 - 3877.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. C. Becker and R. J. Meyer
MobA, the DNA Strand Transferase of Plasmid R1162. THE MINIMAL DOMAIN REQUIRED FOR DNA PROCESSING AT THE ORIGIN OF TRANSFER
J. Biol. Chem., April 19, 2002; 277(17): 14575 - 14580.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
G. Whittle, B. D. Hund, N. B. Shoemaker, and A. A. Salyers
Characterization of the 13-Kilobase ermF Region of the Bacteroides Conjugative Transposon CTnDOT
Appl. Envir. Microbiol., August 1, 2001; 67(8): 3488 - 3495.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
P. S. G. Chain, I. Hernandez-Lucas, B. Golding, and T. M. Finan
oriT-Directed Cloning of Defined Large Regions from Bacterial Genomes: Identification of the Sinorhizobium meliloti pExo Megaplasmid Replicator Region
J. Bacteriol., October 1, 2000; 182(19): 5486 - 5494.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
R. Meyer
Identification of the mob Genes of Plasmid pSC101 and Characterization of a Hybrid pSC101-R1162 System for Conjugal Mobilization
J. Bacteriol., September 1, 2000; 182(17): 4875 - 4881.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.