Nucleic Acids Research, 1990, Vol. 18, No. 12 3587-3596
© 1990
MOLECULAR BIOLOGY |
Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line
Imperial Cancer Research Fund, Lincoln's Inn Fields London WC2A 3PX, UK
*To whom correspondence should be addressed
Received February 19, 1990. Revised May 7, 1990. Accepted May 7, 1990.
We report the development of an advanced system for transfer and expression of exogenous genes in mammalian cells based on Moloney murine leukemia virus (Mo MuLV). Extensive deletion/mutagenesis analysis to identify cis-acting signals involved in virus transmission has led to the design of a family of novel, highly efficient retroviral vectors and a partner helper-free packaging cell line. The pBabe retroviral vector constructs transmit inserted genes at high titres and express them from the Mo MuLV Long Terminal Repeat (LTR). Each of these vectors has been constructed with one of four different dominantly acting selectable markers, allowing the growth of infected mammalian cells in the presence of G418, hygromycin B, bleomycin/phleomycin or puromycin, respectively. The high titre ecotropic helper free packaging cell line, 
E, was designed in conjunction with the pBabe vectors to reduce the risk of generation of wild type Mo MuLV via homologous recombination events. The E cell line was generated with separate gagpol and ecotropic env expression constructs with minimal sequence overlap and decreased sequence homology achieved by ‘codon wobbling’. Homologous env coding sequences were deleted from the pBabe vectors without diminishing recombinant vector titre. Together, the pBabe vectors and E cell line should prove useful in experiments where highest frequencies of gene transfer, or concomitant expression of several different genes within a single cell are required with minimal risk of helper virus contamination.
+Present address: ImmuLogic Pharmaceutical Corp., Bldg. 600, 1 Kendall Sq., Cambridge, MA 02139, USA
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  X. Li, J. Liu, and T. Gao {beta}-TrCP-Mediated Ubiquitination and Degradation of PHLPP1 Are Negatively Regulated by Akt Mol. Cell. Biol., December 1, 2009; 29(23): 6192 - 6205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. D. Jack, L. Zhang, and A. D. Friedman M-CSF elevates c-Fos and phospho-C/EBP{alpha}(S21) via ERK whereas G-CSF stimulates SHP2 phosphorylation in marrow progenitors to contribute to myeloid lineage specification Blood, September 3, 2009; 114(10): 2172 - 2180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gouttenoire, V. Castet, R. Montserret, N. Arora, V. Raussens, J.-M. Ruysschaert, E. Diesis, H. E. Blum, F. Penin, and D. Moradpour Identification of a Novel Determinant for Membrane Association in Hepatitis C Virus Nonstructural Protein 4B J. Virol., June 15, 2009; 83(12): 6257 - 6268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Isaji, Y. Sato, T. Fukuda, and J. Gu N-Glycosylation of the I-like Domain of {beta}1 Integrin Is Essential for {beta}1 Integrin Expression and Biological Function: IDENTIFICATION OF THE MINIMAL N-GLYCOSYLATION REQUIREMENT FOR {alpha}5{beta}1 J. Biol. Chem., May 1, 2009; 284(18): 12207 - 12216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Knauth, E. Cartwright, S. Freund, M. Bycroft, and A. Buchberger VHL Mutations Linked to Type 2C von Hippel-Lindau Disease Cause Extensive Structural Perturbations in pVHL J. Biol. Chem., April 17, 2009; 284(16): 10514 - 10522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Wang, A. Kennedy, P. Das, P. B. McIntosh, S. A. Howell, E. R. Isaacson, S. A. Hinz, C. Davy, and J. Doorbar Phosphorylation of the Human Papillomavirus Type 16 E1^E4 Protein at T57 by ERK Triggers a Structural Change That Enhances Keratin Binding and Protein Stability J. Virol., April 15, 2009; 83(8): 3668 - 3683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Dacwag, M. T. Bedford, S. Sif, and A. N. Imbalzano Distinct Protein Arginine Methyltransferases Promote ATP-Dependent Chromatin Remodeling Function at Different Stages of Skeletal Muscle Differentiation Mol. Cell. Biol., April 1, 2009; 29(7): 1909 - 1921. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Moissoglu, K. S. McRoberts, J. A. Meier, D. Theodorescu, and M. A. Schwartz Rho GDP Dissociation Inhibitor 2 Suppresses Metastasis via Unconventional Regulation of RhoGTPases Cancer Res., April 1, 2009; 69(7): 2838 - 2844. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Gordley, C. A. Gersbach, and C. F. Barbas III Synthesis of programmable integrases PNAS, March 31, 2009; 106(13): 5053 - 5058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. J. Gartner and C. R. Bertozzi Programmed assembly of 3-dimensional microtissues with defined cellular connectivity PNAS, March 24, 2009; 106(12): 4606 - 4610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Lee, B. Bugarija, E. J. Millan, N. M. Walton, J. Gaetz, C. J. Fernandes, W.-H. Yu, N. Mekel-Bobrov, T. W. Vallender, G. E. Snyder, et al. Systematic identification of cis-silenced genes by trans complementation Hum. Mol. Genet., March 1, 2009; 18(5): 835 - 846. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Buschman, P. A. Bromann, P. Cejudo-Martin, F. Wen, I. Pass, and S. A. Courtneidge The Novel Adaptor Protein Tks4 (SH3PXD2B) Is Required for Functional Podosome Formation Mol. Biol. Cell, March 1, 2009; 20(5): 1302 - 1311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wang, V. Patel, H. Miyazaki, J.S. Gutkind, and W.A. Yeudall Role for EPS8 in squamous carcinogenesis Carcinogenesis, January 1, 2009; 30(1): 165 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Weinlander, E. Naschberger, M. H. Lehmann, P. Tripal, W. Paster, H. Stockinger, C. Hohenadl, and M. Sturzl Guanylate binding protein-1 inhibits spreading and migration of endothelial cells through induction of integrin {alpha}4 expression FASEB J, December 1, 2008; 22(12): 4168 - 4178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ji, A. Glaser, M. Wernli, J. M. Berke, D. Moradpour, and P. Erb Suppression of short interfering RNA-mediated gene silencing by the structural proteins of hepatitis C virus J. Gen. Virol., November 1, 2008; 89(11): 2761 - 2766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Zhao, J. Pan, X. Wang, Y. Wu, W. A. Bauman, and C. P. Cardozo Expression of the Muscle Atrophy Factor Muscle Atrophy F-Box Is Suppressed by Testosterone Endocrinology, November 1, 2008; 149(11): 5449 - 5460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ben-Israel, R. Sharf, G. Rechavi, and T. Kleinberger Adenovirus E4orf4 Protein Downregulates MYC Expression through Interaction with the PP2A-B55 Subunit J. Virol., October 1, 2008; 82(19): 9381 - 9388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Possemato, J. C. Timmons, E. L. Bauerlein, N. Wada, A. Baldwin, K. Masutomi, and W. C. Hahn Suppression of hPOT1 in Diploid Human Cells Results in an hTERT-Dependent Alteration of Telomere Length Dynamics Mol. Cancer Res., October 1, 2008; 6(10): 1582 - 1593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ramachandran, J.-P. Parisien, and C. M. Horvath STAT2 Is a Primary Target for Measles Virus V Protein-Mediated Alpha/Beta Interferon Signaling Inhibition J. Virol., September 1, 2008; 82(17): 8330 - 8338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Krawczyk, F. A. Suprynowicz, X. Liu, Y. Dai, D. P. Hartmann, J. Hanover, and R. Schlegel Koilocytosis: A Cooperative Interaction between the Human Papillomavirus E5 and E6 Oncoproteins Am. J. Pathol., September 1, 2008; 173(3): 682 - 688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-X. Ren, F. G. Finckenstein, D. A. Abdueva, V. Shahbazian, B. Chung, K. I. Weinberg, T. J. Triche, H. Shimada, and M. J. Anderson Mouse Mesenchymal Stem Cells Expressing PAX-FKHR Form Alveolar Rhabdomyosarcomas by Cooperating with Secondary Mutations Cancer Res., August 15, 2008; 68(16): 6587 - 6597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-S. Kang, G.-U. Bae, M.-J. Yi, Y.-J. Yang, J.-E. Oh, G. Takaesu, Y. T. Zhou, B. C. Low, and R. S. Krauss A Cdo-Bnip-2-Cdc42 signaling pathway regulates p38{alpha}/{beta} MAPK activity and myogenic differentiation J. Cell Biol., August 11, 2008; 182(3): 497 - 507. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Vitali, C. Mancini, V. Cesi, B. Tanno, M. Mancuso, G. Bossi, Y. Zhang, R. V. Martinez, B. Calabretta, C. Dominici, et al. Slug (SNAI2) Down-Regulation by RNA Interference Facilitates Apoptosis and Inhibits Invasive Growth in Neuroblastoma Preclinical Models Clin. Cancer Res., July 15, 2008; 14(14): 4622 - 4630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Ludlow, M. K. Lo, J. J. Rodriguez, P. A. Rota, and C. M. Horvath Henipavirus V Protein Association with Polo-Like Kinase Reveals Functional Overlap with STAT1 Binding and Interferon Evasion J. Virol., July 1, 2008; 82(13): 6259 - 6271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Rulli Jr., J. Mirro, S. A. Hill, P. Lloyd, R. J. Gorelick, J. M. Coffin, D. Derse, and A. Rein Interactions of Murine APOBEC3 and Human APOBEC3G with Murine Leukemia Viruses J. Virol., July 1, 2008; 82(13): 6566 - 6575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Cao, C. Bao, E. Padalko, and C. J. Lowenstein Acetylation of mitogen-activated protein kinase phosphatase-1 inhibits Toll-like receptor signaling J. Exp. Med., June 9, 2008; 205(6): 1491 - 1503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Basak, V. F.-S. Shih, and A. Hoffmann Generation and Activation of Multiple Dimeric Transcription Factors within the NF-{kappa}B Signaling System Mol. Cell. Biol., May 15, 2008; 28(10): 3139 - 3150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Genovese, N. S. Banerjee, T. R. Broker, and L. T. Chow Casein Kinase II Motif-Dependent Phosphorylation of Human Papillomavirus E7 Protein Promotes p130 Degradation and S-Phase Induction in Differentiated Human Keratinocytes J. Virol., May 15, 2008; 82(10): 4862 - 4873. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Watt, B. J. W. van Denderen, L. A. Castelli, C. R. Bruce, A. J. Hoy, E. W. Kraegen, L. Macaulay, and B. E. Kemp Adipose Triglyceride Lipase Regulation of Skeletal Muscle Lipid Metabolism and Insulin Responsiveness Mol. Endocrinol., May 1, 2008; 22(5): 1200 - 1212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sarmiento, W. Wang, A. Dovas, H. Yamaguchi, M. Sidani, M. El-Sibai, V. DesMarais, H. A. Holman, S. Kitchen, J. M. Backer, et al. WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells J. Cell Biol., March 24, 2008; 180(6): 1245 - 1260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. T. Etheridge, S. A. Compton, K. S. Barrientos, S. Ozgur, J. D. Griffith, and C. M. Counter Tethering Telomeric Double- and Single-stranded DNA-binding Proteins Inhibits Telomere Elongation J. Biol. Chem., March 14, 2008; 283(11): 6935 - 6941. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yano, I. Kobayashi, Y. Onodera, F. Luton, M. Franco, Y. Mazaki, S. Hashimoto, K. Iwai, Z. Ronai, and H. Sabe Fbx8 Makes Arf6 Refractory to Function via Ubiquitination Mol. Biol. Cell, March 1, 2008; 19(3): 822 - 832. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ma, Y. Rong, D. R. Radiloff, M. B. Datto, B. Centeno, S. Bao, A. W. M. Cheng, F. Lin, S. Jiang, T. J. Yeatman, et al. Extracellular matrix protein {beta}ig-h3/TGFBI promotes metastasis of colon cancer by enhancing cell extravasation Genes & Dev., February 1, 2008; 22(3): 308 - 321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Aga-Mizrachi, T. Brutman-Barazani, A. I. Jacob, A. Bak, A. Elson, and S. R. Sampson Cytosolic Protein Tyrosine Phosphatase-{epsilon} Is a Negative Regulator of Insulin Signaling in Skeletal Muscle Endocrinology, February 1, 2008; 149(2): 605 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-S. Gabet, R. Accardi, A. Bellopede, S. Popp, P. Boukamp, B. S. Sylla, J. A. Londono-Vallejo, and M. Tommasino Impairment of the telomere/telomerase system and genomic instability are associated with keratinocyte immortalization induced by the skin human papillomavirus type 38 FASEB J, February 1, 2008; 22(2): 622 - 632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kilbey, K. Blyth, S. Wotton, A. Terry, A. Jenkins, M. Bell, L. Hanlon, E. R. Cameron, and J. C. Neil Runx2 Disruption Promotes Immortalization and Confers Resistance to Oncogene-Induced Senescence in Primary Murine Fibroblasts Cancer Res., December 1, 2007; 67(23): 11263 - 11271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. De Santa, S. Albini, E. Mezzaroma, L. Baron, A. Felsani, and M. Caruso pRb-Dependent Cyclin D3 Protein Stabilization Is Required for Myogenic Differentiation Mol. Cell. Biol., October 15, 2007; 27(20): 7248 - 7265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Masia, S. Alvarez, A. R. de Lera, and D. Barettino Rapid, Nongenomic Actions of Retinoic Acid on Phosphatidylinositol-3-Kinase Signaling Pathway Mediated by the Retinoic Acid Receptor Mol. Endocrinol., October 1, 2007; 21(10): 2391 - 2402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Raz, V. Nardi, M. Azam, J. Cortes, and G. Q. Daley Farnesyl transferase inhibitor resistance probed by target mutagenesis Blood, September 15, 2007; 110(6): 2102 - 2109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Rajashekhar, M. Grow, A. Willuweit, C. E. Patterson, and M. Clauss Divergent and convergent effects on gene expression and function in acute versus chronic endothelial activation Physiol Genomics, September 11, 2007; 31(1): 104 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wang, A. M. Kwok, and J. Y. Chan The p65 Isoform of Nrf1 Is a Dominant Negative Inhibitor of ARE-mediated Transcription J. Biol. Chem., August 24, 2007; 282(34): 24670 - 24678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Corydon, A. Haagerup, T. G. Jensen, H. G. Binderup, M. S. Petersen, K. Kaltoft, J. Vestbo, T. A. Kruse, and A. D. Borglum A functional CD86 polymorphism associated with asthma and related allergic disorders J. Med. Genet., August 1, 2007; 44(8): 509 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Keck, M. K. Summers, D. Tedesco, S. Ekholm-Reed, L.-C. Chuang, P. K. Jackson, and S. I. Reed Cyclin E overexpression impairs progression through mitosis by inhibiting APCCdh1 J. Cell Biol., July 24, 2007; 178(3): 371 - 385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Geiger and D. S. Peeper Critical Role for TrkB Kinase Function in Anoikis Suppression, Tumorigenesis, and Metastasis Cancer Res., July 1, 2007; 67(13): 6221 - 6229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Schelcher, S. Al Mehairi, E. Verrall, Q. Hope, K. Flower, B. Bromley, D. N. Woolfson, M. J. West, and A. J. Sinclair Atypical bZIP Domain of Viral Transcription Factor Contributes to Stability of Dimer Formation and Transcriptional Function J. Virol., July 1, 2007; 81(13): 7149 - 7155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Belguise, S. Guo, and G. E. Sonenshein Activation of FOXO3a by the Green Tea Polyphenol Epigallocatechin-3-Gallate Induces Estrogen Receptor {alpha} Expression Reversing Invasive Phenotype of Breast Cancer Cells Cancer Res., June 15, 2007; 67(12): 5763 - 5770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Hogg and K. Collins RNA-based affinity purification reveals 7SK RNPs with distinct composition and regulation RNA, June 1, 2007; 13(6): 868 - 880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wu, W. Zhao, J. Zhao, J. Pan, Q. Wu, Y. Zhang, W. A. Bauman, and C. P. Cardozo Identification of Androgen Response Elements in the Insulin-Like Growth Factor I Upstream Promoter Endocrinology, June 1, 2007; 148(6): 2984 - 2993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Samson, C. Will, A. Knoblauch, L. Sharek, K. von der Mark, K. Burridge, and V. Wixler Def-6, a Guanine Nucleotide Exchange Factor for Rac1, Interacts with the Skeletal Muscle Integrin Chain {alpha}7A and Influences Myoblast Differentiation J. Biol. Chem., May 25, 2007; 282(21): 15730 - 15742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Chung, K. K. Frese, R. S. Weiss, B. V. V. Prasad, and R. T. Javier A New Crucial Protein Interaction Element That Targets the Adenovirus E4-ORF1 Oncoprotein to Membrane Vesicles J. Virol., May 1, 2007; 81(9): 4787 - 4797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Brass, Z. Pal, N. Sapay, G. Deleage, H. E. Blum, F. Penin, and D. Moradpour Conserved Determinants for Membrane Association of Nonstructural Protein 5A from Hepatitis C Virus and Related Viruses J. Virol., March 15, 2007; 81(6): 2745 - 2757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Sontag, V. Nunbhakdi-Craig, J.-M. Sontag, R. Diaz-Arrastia, E. Ogris, S. Dayal, S. R. Lentz, E. Arning, and T. Bottiglieri Protein Phosphatase 2A Methyltransferase Links Homocysteine Metabolism with Tau and Amyloid Precursor Protein Regulation J. Neurosci., March 14, 2007; 27(11): 2751 - 2759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. A. Hasan, E. Bates, F. Takeshita, A. Biliato, R. Accardi, V. Bouvard, M. Mansour, I. Vincent, L. Gissmann, T. Iftner, et al. TLR9 Expression and Function Is Abolished by the Cervical Cancer-Associated Human Papillomavirus Type 16 J. Immunol., March 1, 2007; 178(5): 3186 - 3197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. D. Sun, M. Malabunga, J. R. Tonra, R. DiRenzo, F. E. Carrick, H. Zheng, H.-R. Berthoud, O. P. McGuinness, J. Shen, P. Bohlen, et al. Monoclonal antibody antagonists of hypothalamic FGFR1 cause potent but reversible hypophagia and weight loss in rodents and monkeys Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E964 - E976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. DeBell, L. Graham, I. Reischl, C. Serrano, E. Bonvini, and B. Rellahan Intramolecular Regulation of Phospholipase C-{gamma}1 by Its C-Terminal Src Homology 2 Domain Mol. Cell. Biol., February 1, 2007; 27(3): 854 - 863. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Puppo, M. C. Bosco, M. Federico, S. Pastorino, and L. Varesio Hypoxia inhibits Moloney murine leukemia virus expression in activated macrophages J. Leukoc. Biol., February 1, 2007; 81(2): 528 - 538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Nie, G. L. Hammond, and J.-H. Yang Double-Stranded RNA Deaminase ADAR1 Increases Host Susceptibility to Virus Infection J. Virol., January 15, 2007; 81(2): 917 - 923. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. B. Deramaudt, M. M. Sachdeva, M. P. Wescott, Y. Chen, D. A. Stoffers, and A. K. Rustgi The PDX1 Homeodomain Transcription Factor Negatively Regulates the Pancreatic Ductal Cell-specific Keratin 19 Promoter J. Biol. Chem., December 15, 2006; 281(50): 38385 - 38395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Paschoud, A. M. Dogar, C. Kuntz, B. Grisoni-Neupert, L. Richman, and L. C. Kuhn Destabilization of Interleukin-6 mRNA Requires a Putative RNA Stem-Loop Structure, an AU-Rich Element, and the RNA-Binding Protein AUF1 Mol. Cell. Biol., November 15, 2006; 26(22): 8228 - 8241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li and S. S. Makarov An essential role of NF-{kappa}B in the "tumor-like" phenotype of arthritic synoviocytes PNAS, November 14, 2006; 103(46): 17432 - 17437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Groisman, M. Ivshina, V. Marin, N. J. Kennedy, R. J. Davis, and J. D. Richter Control of cellular senescence by CPEB Genes & Dev., October 1, 2006; 20(19): 2701 - 2712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Cossins, G. Burke, S. Maxwell, H. Spearman, S. Man, J. Kuks, A. Vincent, J. Palace, C. Fuhrer, and D. Beeson Diverse molecular mechanisms involved in AChR deficiency due to rapsyn mutations Brain, October 1, 2006; 129(10): 2773 - 2783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nedachi and M. Kanzaki Regulation of glucose transporters by insulin and extracellular glucose in C2C12 myotubes Am J Physiol Endocrinol Metab, October 1, 2006; 291(4): E817 - E828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Gallagher, S. Hoffmann, N. Brown, A. Cedzich, S. Meruvu, D. Podlich, Y. Feng, V. Konecke, U. de Vries, H.-P. Hammes, et al. A Truncated Polycystin-2 Protein Causes Polycystic Kidney Disease and Retinal Degeneration in Transgenic Rats J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2719 - 2730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Hassan, S. J. Simmonds, N. G. Clarkson, S. Hanrahan, M. J. Puklavec, M. Bomb, A. N. Barclay, and M. H. Brown CD6 Regulates T-Cell Responses through Activation-Dependent Recruitment of the Positive Regulator SLP-76. Mol. Cell. Biol., September 1, 2006; 26(17): 6727 - 6738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. McCown and A. Pekosz Distinct domains of the influenza a virus m2 protein cytoplasmic tail mediate binding to the m1 protein and facilitate infectious virus production. J. Virol., August 1, 2006; 80(16): 8178 - 8189. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Patwardhan, Y. Shen, G. S. Goldberg, and W. T. Miller Individual Cas Phosphorylation Sites Are Dispensable for Processive Phosphorylation by Src and Anchorage-independent Cell Growth J. Biol. Chem., July 28, 2006; 281(30): 20689 - 20697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. L. de la Serna, Y. Ohkawa, C. Higashi, C. Dutta, J. Osias, N. Kommajosyula, T. Tachibana, and A. N. Imbalzano The Microphthalmia-associated Transcription Factor Requires SWI/SNF Enzymes to Activate Melanocyte-specific Genes J. Biol. Chem., July 21, 2006; 281(29): 20233 - 20241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-T. Chou and Y.-C. Yang Post-transcriptional Control of Cited2 by Transforming Growth Factor beta: REGULATION VIA SMADS AND CITED2 CODING REGION J. Biol. Chem., July 7, 2006; 281(27): 18451 - 18462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Banerjee, N. J. Genovese, F. Noya, W.-M. Chien, T. R. Broker, and L. T. Chow Conditionally activated e7 proteins of high-risk and low-risk human papillomaviruses induce s phase in postmitotic, differentiated human keratinocytes. J. Virol., July 1, 2006; 80(13): 6517 - 6524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. B. Deramaudt, M. Takaoka, R. Upadhyay, M. J. Bowser, J. Porter, A. Lee, B. Rhoades, C. N. Johnstone, R. Weissleder, S. R. Hingorani, et al. N-Cadherin and Keratinocyte Growth Factor Receptor Mediate the Functional Interplay between Ki-RASG12V and p53V143A in Promoting Pancreatic Cell Migration, Invasion, and Tissue Architecture Disruption. Mol. Cell. Biol., June 1, 2006; 26(11): 4185 - 4200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mattiussi, K. Matsumoto, B. Illi, F. Martelli, M. C. Capogrossi, and C. Gaetano Papilloma protein E6 abrogates shear stress-dependent survival in human endothelial cells: Evidence for specialized functions of paxillin Cardiovasc Res, June 1, 2006; 70(3): 578 - 588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wang, G. Mouneimne, M. Sidani, J. Wyckoff, X. Chen, A. Makris, S. Goswami, A. R. Bresnick, and J. S. Condeelis The activity status of cofilin is directly related to invasion, intravasation, and metastasis of mammary tumors J. Cell Biol., May 8, 2006; 173(3): 395 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Li, B. Chang, M. Imamura, N. Poungvarin, and L. Chan Glucose-dependent transcriptional regulation by an evolutionarily conserved glucose-sensing module. Diabetes, May 1, 2006; 55(5): 1179 - 1189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gregor, A. Zeold, S. Oehler, K. A. Marobela, P. Fuchs, G. Weigel, D. G. Hardie, and G. Wiche Plectin scaffolds recruit energy-controlling AMP-activated protein kinase (AMPK) in differentiated myofibres J. Cell Sci., May 1, 2006; 119(9): 1864 - 1875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gray, M. J. Churchill, N. Keane, J. Sterjovski, A. M. Ellett, D. F. J. Purcell, P. Poumbourios, C. Kol, B. Wang, N. K. Saksena, et al. Genetic and Functional Analysis of R5X4 Human Immunodeficiency Virus Type 1 Envelope Glycoproteins Derived from Two Individuals Homozygous for the CCR5{Delta}32 Allele. J. Virol., April 1, 2006; 80(7): 3684 - 3691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zebisch, P. B. Staber, A. Delavar, C. Bodner, K. Hiden, K. Fischereder, M. Janakiraman, W. Linkesch, H. W. Auner, W. Emberger, et al. Two Transforming C-RAF Germ-Line Mutations Identified in Patients with Therapy-Related Acute Myeloid Leukemia. Cancer Res., April 1, 2006; 66(7): 3401 - 3408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Pennini, R. K. Pai, D. C. Schultz, W. H. Boom, and C. V. Harding Mycobacterium tuberculosis 19-kDa Lipoprotein Inhibits IFN-{gamma}-Induced Chromatin Remodeling of MHC2TA by TLR2 and MAPK Signaling J. Immunol., April 1, 2006; 176(7): 4323 - 4330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-T. Kuan, K. Wakiya, J. M. Dowell, J. E. Herndon II, D. A. Reardon, M. W. Graner, G. J. Riggins, C. J. Wikstrand, and D. D. Bigner Glycoprotein nonmetastatic melanoma protein B, a potential molecular therapeutic target in patients with glioblastoma multiforme. Clin. Cancer Res., April 1, 2006; 12(7): 1970 - 1982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kobayashi, T. Wang, M. Maezawa, M. Kobayashi, S. Ohnishi, K. Hatanaka, S. Hige, Y. Shimizu, M. Kato, M. Asaka, et al. Overexpression of the Oncoprotein Prothymosin {alpha} Triggers a p53 Response that Involves p53 Acetylation. Cancer Res., March 15, 2006; 66(6): 3137 - 3144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Jounaidi, C.-S. Chen, G. J. Veal, and D. J. Waxman Enhanced antitumor activity of P450 prodrug-based gene therapy using the low Km cyclophosphamide 4-hydroxylase P450 2B11. Mol. Cancer Ther., March 1, 2006; 5(3): 541 - 555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Takaoka, C. E. Smith, M. K. Mashiba, T. Okawa, C. D. Andl, W. S. El-Deiry, and H. Nakagawa EGF-mediated regulation of IGFBP-3 determines esophageal epithelial cellular response to IGF-I Am J Physiol Gastrointest Liver Physiol, February 1, 2006; 290(2): G404 - G416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. K. Henry, J. R. Field, E. M. Adkins, M. L. Parnas, R. A. Vaughan, M.-F. Zou, A. H. Newman, and R. D. Blakely Tyr-95 and Ile-172 in Transmembrane Segments 1 and 3 of Human Serotonin Transporters Interact to Establish High Affinity Recognition of Antidepressants J. Biol. Chem., January 27, 2006; 281(4): 2012 - 2023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Boyd, Y.-Y. Xiao, K. Fan, A. Poholek, N. G. Copeland, N. A. Jenkins, and A. S. Perkins Sox4 cooperates with Evi1 in AKXD-23 myeloid tumors via transactivation of proviral LTR Blood, January 15, 2006; 107(2): 733 - 741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gebhardt, M. Frye, S. Herold, S. A. Benitah, K. Braun, B. Samans, F. M. Watt, H.-P. Elsasser, and M. Eilers Myc regulates keratinocyte adhesion and differentiation via complex formation with Miz1 J. Cell Biol., January 3, 2006; 172(1): 139 - 149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Silla, I. Haal, J. Geimanen, K. Janikson, A. Abroi, E. Ustav, and M. Ustav Episomal Maintenance of Plasmids with Hybrid Origins in Mouse Cells J. Virol., December 15, 2005; 79(24): 15277 - 15288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Guess and D. J. McCance Decreased Migration of Langerhans Precursor-Like Cells in Response to Human Keratinocytes Expressing Human Papillomavirus Type 16 E6/E7 Is Related to Reduced Macrophage Inflammatory Protein-3{alpha} Production J. Virol., December 1, 2005; 79(23): 14852 - 14862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Nadella and L. S. Kirschner Disruption of Protein Kinase A Regulation Causes Immortalization and Dysregulation of D-Type Cyclins Cancer Res., November 15, 2005; 65(22): 10307 - 10315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Freche, P. Guillaumot, J. Charmetant, L. Pelletier, C. Luquain, D. Christiansen, M. Billaud, and S. N. Manie Inducible Dimerization of RET Reveals a Specific AKT Deregulation in Oncogenic Signaling J. Biol. Chem., November 4, 2005; 280(44): 36584 - 36591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Graham, A. C. Sims, S. M. Brockway, R. S. Baric, and M. R. Denison The nsp2 Replicase Proteins of Murine Hepatitis Virus and Severe Acute Respiratory Syndrome Coronavirus Are Dispensable for Viral Replication J. Virol., November 1, 2005; 79(21): 13399 - 13411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Schelcher, S. Valencia, H.-J. Delecluse, M. Hicks, and A. J. Sinclair Mutation of a Single Amino Acid Residue in the Basic Region of the Epstein-Barr Virus (EBV) Lytic Cycle Switch Protein Zta (BZLF1) Prevents Reactivation of EBV from Latency J. Virol., November 1, 2005; 79(21): 13822 - 13828. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Gordon, N. Alon, and M. Buchwald FANCC, FANCE, and FANCD2 Form a Ternary Complex Essential to the Integrity of the Fanconi Anemia DNA Damage Response Pathway J. Biol. Chem., October 28, 2005; 280(43): 36118 - 36125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Barnes, D. Palaiologou, E. Panousopoulou, B. Schultheis, A. S.M. Yong, A. Wong, L. Pattacini, J. M. Goldman, and J. V. Melo Bcr-Abl Expression Levels Determine the Rate of Development of Resistance to Imatinib Mesylate in Chronic Myeloid Leukemia Cancer Res., October 1, 2005; 65(19): 8912 - 8919. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Yatsula, S. Lin, A. J. Read, A. Poholek, K. Yates, D. Yue, P. Hui, and A. S. Perkins Identification of Binding Sites of EVI1 in Mammalian Cells J. Biol. Chem., September 2, 2005; 280(35): 30712 - 30722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Cleasby, J. R. Davey, T. A. Reinten, M. W. Graham, D. E. James, E. W. Kraegen, and G. J. Cooney Acute Bidirectional Manipulation of Muscle Glucose Uptake by In Vivo Electrotransfer of Constructs Targeting Glucose Transporter Genes Diabetes, September 1, 2005; 54(9): 2702 - 2711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Boehm, M. T. Hession, S. E. Bulmer, and W. C. Hahn Transformation of Human and Murine Fibroblasts without Viral Oncoproteins Mol. Cell. Biol., August 1, 2005; 25(15): 6464 - 6474. [Abstract] [Full Text] [PDF]
|
|