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Nucleic Acids Research, 1990, Vol. 18, No. 13 3697-3703
© 1990

GENOME STRUCTURE AND MAPPING

The human interleukin-2 receptor ß-chain gene: genomic organization, promoter analysis and chromosomal assignment

Hiroshi Shibuya*, Mitsutoshi Yoneyama, Yusuke Nakamura1,+, Hisashi Harada, Masanori Hatakeyama, Seijiro Minamoto, Takeshi Kono, Takeshi Doi, Raymond White1 and Tadatsugu Taniguchi

Institute for Molecular and Cellular Biology, Osaka University 1-3 Yamadaoka, Suita-shi, Osaka 565, Japan 1Howard Hughes Medical Institute, Research Laboratories, University of Utah 701 Wintrobe Building, Room 631, Salt Lake City, UT 84132, USA

*To whom correspondence should be addressed

Received May 18, 1990. Accepted June 6, 1990.

The chromosomal gene for the human interleukin-2 receptor ß-chain (IL-2Rß) was isolated and characterized. The entire IL-2Rß3 gene is composed of ten exons spanning about 24.3 kilobases, in which the protein is encoded by the exons 2–10. The cysteine rich extracellular region which displays a significant evolutionary resemblance to other cytokine receptors, as well as growth hormone and prolactin receptors, is encoded primarily by exons 3 and 4, whereas the membrane proximal, cysteine poor domain showing a homology with type III modules of fibronectin is encoded by exon 7. Sequence analysis of the 5'-flanking region revealed the presence of potential binding sites for transcription factors such as Octamer binding factors, AP-1, AP-2 as well as the ‘GC-clusters’. At least five potential cap sites were identified by S1 mapping analysis. The 850 bp DNA sequence of the 5'-flanking region exhibited constitutive promoter activity when it was linked upstream of the HSV-tk reporter gene and then transfected into YT cells, a human leukemic cell line. By applying the RFLP linkage analysis, the IL-2Rß gene has been assigned to chromosome 22q12–13.

+Present address: Department of Biochemistry. Cancer Institute Japanese Foundation for Cancer Research, Toshima-ku, Tokyo 170, Japan


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