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Nucleic Acids Research, 1990, Vol. 18, No. 13 3813-3821
© 1990

CHEMISTRY

An improved procedure for derivatization of controlled-pore glass beads for solid-phase oligonucleotide synthesis

Masad J. Damha*, Paul A. Giannaris and Steven V. Zabarylo

Department of Chemistry, Erindale College, University of Toronto Mississauga, Ontario L5L 1C6, Canada

*To whom correspondence should be addressed

Received April 9, 1990. Revised May 17, 1990. Accepted May 17, 1990.

A simplified and economical method for the attachment of 2'-deoxyribo, ribo and arabinonucleosides onto long-chain alkylamidopropanoic acid controlled-pore glass (LCAAP-CPG, P-3) is described. In this procedure, 5'-0-tritylated nucleosides are coupled directly to LCAAP-CPG in excellent yields using 1-(3-dimethylamino-propyl)-3-ethylcarbodiimide (DEC) as coupling reagent. The conventional and time-consuming preparation of nucleoside-3'-O-succinates is no longer required.


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