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Nucleic Acids Research, 1990, Vol. 18, No. 13 3983-3986
© 1990

MOLECULAR BIOLOGY

Constructing DNA by polymerase recombination

A.A. Yolov and Z.A. Shabarova

Department of Chemistry, M.V.Lomonosov State University Moscow 117234, USSR

Received November 23, 1989. Revised February 20, 1990. Accepted February 20, 1990.

Polymerase-mediated recombination based on DNA polymerase chain reactions (PCRs) has been used to carry out directed joining at a preset point of two DNA fragments initially contained in a plasmid and a single-stranded synthetic DNA. The process includes copying of these fragments by PCR with generation of an overlapping homologous region. Such overlap of 12 base pairs in length was found to be sufficient to provide further DNA joining also by use of PCR.


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