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Nucleic Acids Research, 1990, Vol. 18, No. 14 4067
© 1990

MOLECULAR BIOLOGY

DNA-sequence and metal-ion specificity of the formation of *H-DNA

J. Bernués, R. Beltrán, J.M. Casasnovas and F. Azorín*

Grupo de Química Macromolecular, Centro de Investigation y Desarrollo CSIC, ETSEIB Diagonal 647, 08028 Barcelona, Spain

*To whom correspondence should be addressed

Received May 22, 1990. Accepted June 13, 1990.

The homopyrimidine-homopurine sequence d(CT/GA)22 undergoes, in the presence of zinc ions, transition to an altered DNA conformation (*H-DNA) which is neither H-DNA nor B-DNA. *H-DNA is characterized by a peculiar chemical reactivity pattern in which most of the polypyrimidine strand is hyperreactive to osmium tetroxide and the central part of the polypurine strand is sensitive to diethylpyrocarbonate. Formation of *H-DNA is specific of metal-ion. *H-DNA is detected in the presence of Zn++, Cd++ and Mn++. The efficiency on promoting the transition is in the order of Zn ++ > Cd++ >> Mn++. Formation of *H-DNA is also specific of nucleotide sequence. From all the different homopolymeric sequences tested only the d(CT/GA)22 sequence showed the zinc-induced transition to *H-DNA. These results suggest that stabilization of *H-DNA involves the formation of a specific complex between the metal-ion and the nucleotide sequence. The biological relevance of these results is discussed in view of the important role that zinc ions play on many nucleic acids processes.


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