Nucleic Acids Research, 1990, Vol. 18, No. 15 4401-4407
© 1990
MOLECULAR BIOLOGY |
Characterization of the human spr2 promoter: induction after UV irradiation or TPA treatment and regulation during differentiation of cultured primary keratinocytes
Laboratory of Molecular Genetics (RCG), Department of Biochemistry, University of Leiden, Gorlaeus Laboratories Einsteinweg 5, PO Box 9502, 2300 RA Leiden, The Netherlands
*To whom correspondence should be addressed
Received May 17, 1990. Accepted July 6, 1990.
We have isolated genomic clones from several members of the UV and TPA inducible human spr2 gene-family in order to analyse the regulation of these genes at a molecular level. From one of these members, the spr2–1 gene, we have identified and sequenced the regulatory region. By using CAT fusion plasmids and a liposome mediated transfection procedure we show that the isolated promoter region contains all the cis-elements necessary for induced expression after UV irradiation or phorbolester treatment of cultured human keratinocytes. Additionally the spr2–1 promoter is shown to be regulated aswell during the normal process of keratinocyte differentiation. This makes the spr2–1 promoter sequence an ideal tool to study the molecular mechanisms by which environmental agents such as UV radiation and chemical tumor promoters interfere with normal gene expression during cell proliferation and differentiation.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Cabral, A. Sayin, S. de Winter, D. F. Fischer, S. Pavel, and C. Backendorf SPRR4, a novel cornified envelope precursor: UV-dependent epidermal expression and selective incorporation into fragile envelopes J. Cell Sci., January 11, 2001; 114(21): 3837 - 3843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Hildesheim, U Kuhn, C. Yee, R. Foster, K. Yancey, and J. Vogel The hSkn-1a POU transcription factor enhances epidermal stratification by promoting keratinocyte proliferation J. Cell Sci., January 5, 2001; 114(10): 1913 - 1923. [Abstract] [PDF]
|
|
|
|
 |
|
 S.-I. Jang, N. Karaman-Jurukovska, M. I. Morasso, P. M. Steinert, and N. G. Markova Complex Interactions between Epidermal POU Domain and Activator Protein 1 Transcription Factors Regulate the Expression of the Profilaggrin Gene in Normal Human Epidermal Keratinocytes J. Biol. Chem., May 12, 2000; 275(20): 15295 - 15304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hildesheim, R. A. Foster, M. E. Chamberlin, and J. C. Vogel Characterization of the Regulatory Domains of the Human Skn-1a/Epoc-1/Oct-11 POU Transcription Factor J. Biol. Chem., September 10, 1999; 274(37): 26399 - 26406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Candi, E. Tarcsa, W. W. Idler, T. Kartasova, L. N. Marekov, and P. M. Steinert Transglutaminase Cross-linking Properties of the Small Proline-rich 1 Family of Cornified Cell Envelope Proteins. INTEGRATION WITH LORICRIN J. Biol. Chem., March 12, 1999; 274(11): 7226 - 7237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Medvedev, N. A. Saunders, H. Matsuura, A. Chistokhina, and A. M. Jetten Regulation of the Transglutaminase I Gene. IDENTIFICATION OF DNA ELEMENTS INVOLVED IN ITS TRANSCRIPTIONAL CONTROL IN TRACHEOBRONCHIAL EPITHELIAL CELLS J. Biol. Chem., February 5, 1999; 274(6): 3887 - 3896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. J. Sark, D. F. Fischer, E. de Meijer, P. van de Putte, and C. Backendorf AP-1 and Ets Transcription Factors Regulate the Expression of the Human SPRR1A Keratinocyte Terminal Differentiation Marker J. Biol. Chem., September 18, 1998; 273(38): 24683 - 24692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Tarcsa, E. Candi, T. Kartasova, W. W. Idler, L. N. Marekov, and P. M. Steinert Structural and Transglutaminase Substrate Properties of the Small Proline-rich 2 Family of Cornified Cell Envelope Proteins J. Biol. Chem., September 4, 1998; 273(36): 23297 - 23303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Austin, W. Fujimoto, K. W. Marvin, T. M. Vollberg, L. Lorand, and A. M. Jetten Cloning and Regulation of Cornifin beta, a New Member of the Cornifin/spr Family J. Biol. Chem., February 16, 1996; 271(7): 3737 - 3742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. W. Marvin, W. Fujimoto, and A. M. Jetten Identification and Characterization of a Novel Squamous Cell-associated Gene Related to PMP22 J. Biol. Chem., December 1, 1995; 270(48): 28910 - 28916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. M. Reddy, Y.-J. Chuu, P. N. Lao, J. Donn, D. K. Ann, and R. Wu Expression of Human Squamous Cell Differentiation Marker, SPR1, in Tracheobronchial Epithelium Depends on JUN and TRE Motifs J. Biol. Chem., November 3, 1995; 270(44): 26451 - 26459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cabral, P. Voskamp, A.-M. Cleton-Jansen, A. South, D. Nizetic, and C. Backendorf Structural Organization and Regulation of the Small Proline-rich Family of Cornified Envelope Precursors Suggest a Role in Adaptive Barrier Function J. Biol. Chem., May 25, 2001; 276(22): 19231 - 19237. [Abstract] [Full Text] [PDF]
|
|