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Nucleic Acids Research, 1990, Vol. 18, No. 15 4499-4506
© 1990

MOLECULAR BIOLOGY

The most abundant nascent poly(A)+RNAs are transcribed by RNA polymerase III in murine tumor cells

D.A. Kramerov, S.V. Tillib, G.P. Shumyatsky and G.P. Georgiev

Engelhardt Institute of Molecular Biology, USSR Academy of Sciences Vavilov Street 32, Moscow B-334, USSR

Received April 6, 1990. Revised June 19, 1990. Accepted June 19, 1990.

Twelve to twenty percent of newly synthesized poly(A) + RNA is transcribed by RNA polymerase III in Ehrlich ascites carcinoma and P3O1 plasmocytoma mouse tumors. Most of this RNA designated as pol lllpoly(A) + RNA has a size of 160 to 800 nucleotides with a maximum of distribution of ca. 300 nucleotides. Pol lllpoly(A) + RNA fraction consists of two major classes of molecules corresponding to previously described B1 RNA and B2 RNA with the ratio of 1:4 to 2:3. All B2 RNAs present in poly(A)+ fraction contain a long poly(A) segments at the 3' ends. Thus, RNA polymerase III transcripts can be polyadenylated. Several transcripts that hybridize with B2 probe were also observed in poly(A)–RNA. The major components consist of 180, 160, 120 and 95 nucleotides. The 180-nucleotide B2 RNA seems to be a primary transcript from B2 repeat. We suggest that other B2 RNAs are transcribed from truncated copies of B2 element.


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