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Nucleic Acids Research, 1990, Vol. 18, No. 15 4507-4512
© 1990

MOLECULAR BIOLOGY

The complete nucleotide sequence of PEBV RNA2 reveals the presence of a novel open reading frame and provides insights into the structure of tobraviral subgenomic promoters

M.G. Goulden*, G.P. Lomonossoff, J.W. Davies and K.R. Wood1

Department of Virus Research, John Innes Institute for Plant Science Research Colney Lane, Norwich NR4 7UH

*To whom correspondence, should be addressed at: The Sainsbury Laboratory, John Innes Institute for Plant Science Research, Colney Lane, Norwich NR4 7UH, UK

Received April 5, 1990. Revised June 28, 1990. Accepted June 28, 1990.

The 3374 nucleotide sequence of RNA2 from the British PEBV strain SP5 has been determined. The RNA includes three open reading frames flanked by 5' and 3' noncoding regions of 509 and 480 nucleotides. The open reading frames specify coat protein, a 29.6K product homologous to the 29.1 K product of TRVfTCM) RNA2 and a 23K product not homologous to any previously described protein. The homology demonstrated between the coat proteins of PRV, TRV and PEBV indicates a common evolutionary origin for these proteins. Upstream of each ORF are located sequences homologous to those with which subgenomic RNAs of other tobraviruses start. Subgenomic RNAs for the expression of the three ORFs may start at these points. On all five tobraviral RNA2 molecules sequenced to date, these sequences were found upstream of the coat protein ORF in association with a strongly-conserved potential secondary structural element. Similar potential structures were identified upstream of other tobraviral ORFs. These structures may contribute to the activity of the tobraviral subgenomic promoter.

1department of Microbiology, University of Birmingham, Birmingham B15 2TT, UK


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