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Nucleic Acids Research, 1990, Vol. 18, No. 16 4659-4664
© 1990

Articles

Cloning and nucleotide sequence of the genes coding for the Sau961 restriction and modification enzymes

László Szilák+, Pál Venetianer and Antal Kiss*

Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences PO Box 521, 6701 Szeged, Hungary

*To whom correspondence should be addressed

Received June 29, 1990. Revised July 23, 1990. Accepted July 23, 1990.

The genes coding for the GGNCC specific Sau96l restriction and modification enzymes were cloned and expressed in E. coli. The DNA sequence predicts a 430 amino acid protein (Mr: 49,252) for the methyltransferase and a 261 amino acid protein (Mr: 30,486) for the endonuclease. No protein sequence similarity was detected between the Sau961 methyltransferase and endonuclease. The methyltransferase contains the sequence elements characteristic for m5C-methyltransferases. In addition to this, M.Sau961 shows similarity, also in the variable region, with one m5C-methyltransferase (M.Sinl) which has closely related recognition specificity (GGA/TCC). M.Sau96l methylates the internal cytosine within the GGNCC recognition sequence. The Sau96l endonuclease appears to act as a monomer.

+Permanent address: Agricultural Biotechnology Center, POB 170, 2100 G6d6116, Hungary


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