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Nucleic Acids Research, 1990, Vol. 18, No. 16 4727-4736
© 1990

Articles

An undecamer DNA sequence directs termination of human ribosomal gene transcription

Christa Pfleiderer, Amke Smid, Ingrid Bartsch and Ingrid Grummt*

Institut für Biochemie, Rontgenring 11 8700 Würzburg, FRG

*To whom correspondence should be addressed

Received June 5, 1990. Revised July 19, 1990. Accepted July 19, 1990.

Previously we have shown that a repetitive 18 bp sequence motif, the Sal box (AGGTCGACC-AGA/TT/ANTCCG), present in the 3' terminal spacer ofmouse rDNA constitutes a termination signal for RNA polymerase I (pol I). Similar sequence elements whichare functionally analogous to the murine terminator are present in the spacer of human rDNA. However, the human termination signal is shorter encompassing only 11 bp (GGGTCGACCAG) which correspond to the proximal part of the mouse sequence. Two out of the five human Sal box elements are functionally inactive due to natural point mutations which damage factor binding. A similar sequence motif with a 10 of 11 base identity with the downstream terminators is located upstream of the human transcription initiation site. The upstream element interacts with the same factor(s) asthe downstream terminators and is also capable to stop elongating human RNA polymerase I. Despite the human and mouse factors exert different electrophoretic mobilities in gel retardation assays, UV-crosslinking and proteolytic clipping experiments indicate that both the sizes and the tertiary structure of the Sal box binding proteins of both species are very similar. When bound to DNA, both the human and themouse factor terminate transcription of pol I from the heterologous species. The results implicate that changes in signal sequences necessary for termination have been accompanied by compensatory changes inthe DNA binding domain of the protein(s) interacting with the termination signal. In contrast, the protein-protein interactions between the termination factor and the transcribing RNA polymerase I appear to have been conserved during evolution.


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