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Nucleic Acids Research, 1990, Vol. 18, No. 16 4883-4890
© 1990

MOLECULAR BIOLOGY

Crosslinking of tRNA containing a long extra arm to elongation factor Tu by trans-diamminedichloroplatinum(II)

Nils-Jørgen Rasmussen, Friedrik P. Wikman and B.F.C. Clark

Department of Biostructural Chemistry, Institute of Chemistry, University of Aarhus Denmark

Received March 29, 1990. Revised July 17, 1990. Accepted July 17, 1990.

A tRNA containing a long extra arm, namely E. coli tRNA1leu has been crosslinked to elongation factor Tu, with the crosslinking reagent trans-diamminedichloroplatinum(II). The nucleotide involved in the crosslinking was identified to be a guanosine in the variable region at position 47F or 47G.


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