Nucleic Acids Research, 1990, Vol. 18, No. 17 5019-5026
© 1990
MOLECULAR BIOLOGY |
Improved detection of helix-turn-helix DNA-binding motifs in protein sequences
Department of Biochemistry, University of Adelaide Box 498 GPO, Adelaide SA 5001, Austraiia
Received July 3, 1990. Revised August 8, 1990. Accepted August 8, 1990.
We present an update of our method for systematic detection and evaluation of potential helix-turn-helix DNA-binding motifs in protein sequences [Dodd, I. and Egan, J. B. (1987) J. Mol. Biol. 194, 557–564]. The new method is considerably more powerful, detecting approximately 50% more likely helix-turn-helix sequences without an increase in false predictions. This improvement is due almost entirely to the use of a much larger reference set of 91 presumed helix-turn-helix sequences. The scoring matrix derived from this reference set has been calibrated against a large protein sequence database so that the score obtained by a sequence can be used to give a practical estimation of the probability that the sequence is a helix-turn-helix motif.
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