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Nucleic Acids Research, 1990, Vol. 18, No. 17 5089-5095
© 1990

MOLECULAR BIOLOGY

The Trypanosoma brucei protein phosphatase gene: polycistronic transcription with the RNA polymerase II largest subunit gene

Raymond Evers and Albert W.C.A. Cornelissen*

Max-Planck-Institut für Biologie, Molecular Parasitology Unit Spemannstrasse 34, 7400 Tübingen, FRG

*To whom correspondence should be addressed

Received June 11, 1990. Revised July 30, 1990. Accepted July 30, 1990.

We have previously described the trypanosomal gene encoding the largest subunit of RNA polymerase II (RNAP II) and found that two almost identical genes are encoded within the Trypanosoma brucei genome. Here we show by Southern analyses that the 5' breakpoint between both loci is located approximately 7.5 kb upstream of the RNAP II genes. Northern analyses revealed that the 5' duplicated segment contains at least four other genes, which are transcribed in both bloodstream and procyclic trypanosomes. The gene located immediately upstream of the RNAP II gene in both loci was characterized by sequence analyses. The deduced amino acid sequences show a high degree of similarity to the catalytic subunit of protein phosphatase class 1 (PP1) genes. S1 mapping provided strong evidence in support of the fact that the PP1 and RNAP II genes belong to a single transcription unit.


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