Nucleic Acids Research, 1990, Vol. 18, No. 17 5133-5141
© 1990
MOLECULAR BIOLOGY |
Nuclear pre-mRNA introns: analysis and comparison of intron sequences from Tetrahymena thermophila and other eukaryotes
Department of Microbiology, Macdonald College of McGill University 21 111 Lakeshore Road, Ste Anne de Bellevue, PQ H9X 1C0, Canada
Received May 30, 1990. Revised July 30, 1990. Accepted July 30, 1990.
We have sequenced 14 introns from the ciliate Tetrahymena thermophila and include these in an analysis of the 27 intron sequences available from seven T. thermophila protein-encoding genes. Consensus 5' and 3' splice junctions were determined and found to resemble the junctions of other nuclear pre-mRNA introns. Unique features are noted and discussed. Overall the introns have a mean A+T content of 85% (21% higher than neighbouring exons) with smaller introns tending towards a higher A + T content. Approximately half of the introns are less than 100 bp. Introns from other organisms (approximately 30 for each) were also examined. The introns of Dictyostelium discoideum, Caenorhabditis elegans and Drosophila melanogaster, like those of T. thermophila, have a much higher mean A+T content than their neighbouring exons (>20%). Introns from plants, Neurospora crassa and Schizosaccharomyces pombe also have a significantly higher A+T content (10%–20%). Since a high A+T content is required for intron splicing in plants (58), the elevated A+T content in the introns of these other organisms may also be functionally significant. The introns of yeast (Saccharomyces cerevisiae) and mammals (humans) appear to lack this trait and thus in some aspects may be atypical. The polypyrimidine tract, so distinctive of vertebrate introns, is not a trait of the introns in the non-vertebrate organisms examined in this study.
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