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Nucleic Acids Research, 1990, Vol. 18, No. 17 5219-5225
© 1990

MOLECULAR BIOLOGY

Molecular cloning and expression of four actin isoforms during Artemiadevelopment

Maria-Teresa Macias and Leandro Sastre

Instituto de Investigaciones Biomédicas del CSIC C/Arturo Duperier 4, 28029 Madrid, Spain

Received May 2, 1990. Revised August 8, 1990. Accepted August 8, 1990.

Four cDNA clones coding for different Artemia actin isoforms have been isolated. Three of the clones contain the complete coding sequences while the fourth one lacks 145 bases, coding for the 49 amino terminal amino acids of the protein. The amino acid sequences predicted for the four actin isoforms identified are highly homologous to insect actins as well as to vertebrate cytoplasmic actins. The four identified cDNA clones code for mRNAs of 5.2, 1.9, 1.6 and 1.8 kb, respectively, whose expression is regulated during development. Three of the actin mRNAs are present in cryptobiotic embryos while the other is not. The steady-state levels of all four mRNAs increase during development to reach maximal levels by 10 – 15 hours of development and decrease thereafter. The total number of actin genes encoded in the Artemia genome has been estimated as 8 to 10 by Southern analysis of total DNA.


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