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Nucleic Acids Research, 1990, Vol. 18, No. 18 5529-5532
© 1990

MOLECULAR BIOLOGY

Deletion of human JK segments by site-specific recombination recognizing the conserved nonamer and heptamer sequences

Tomoyuki Nakatani, Kazuhiko Horigome, Noriko Nomura, Tetsuya Kondo, Hiroshi Ohtsuka, Hiroshi Noguchi* and Tasuku Honjo1

Sumitomo Chemical Co., Ltd., Takarazuka Research Center, Laboratory of Biotechnology 4-2-1, Takatsukasa, Takarazuka, Hyogo 665 1Department of Medical Chemistry, Kyoto University Faculty of Medicine Yoshida, Sakyo-ku, Kyoto 606, Japan

*To whom correspondence should be addressed

Received May 3, 1990. Revised August 28, 1990. Accepted August 28, 1990.

Mapping and partial sequencing of the productive K chain genomic DNA of FK-001 demonstrated a 1.8-kb deletion including the JK2, JK3, JK4, and JK5 segments. This deletion occurred between the heptamer recombination signal sequence of the JK2 segment and the heptamer-like sequence located 1.8 kb downstream of the JK2 segment. The recombination reaction kept the reciprocally joined signal sequences on the chromosome and deleted the intervening DNA segment. The cloned FK-001 K chain gene was expressed efficiently in mouse myeloma cells, demonstrating that the 1.8-kb deleted region conferred no functions for gene expression.


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