Nucleic Acids Research, 1990, Vol. 18, No. 19 5767-5774
© 1990
MOLECULAR BIOLOGY |
Chromatin assembly on replicating DNA in vitro
1Laboratory of Molecular Biology, NIDDK, National Institutes of Health Bethesda, MD 20892, USA 2Institut Jacques Monod, CNRS, Université Paris 7, Tour 43, 2 Place Jussieu F-75251, Paris Cedex 05, France
*To whom correspondence should be addressed
Received June 13, 1990. Revised August 21, 1990. Accepted August 21, 1990.
Replicating single-stranded DNA is preferentially assembled Into chromatin in Xenopus egg extracts relative to non-replicating double-stranded DNA. We have examined the molecular basis of this phenomenon. Single-stranded DNA itself is not a favored template for nucleosome assembly in comparison to double-stranded DNA. Complementary strand synthesis is required for the rapid assembly of nucleosomes. We present evidence that the assembly of chromatin on replicating DNA is a two step phenomenon. The first step involves the replication of DNA and the assembly of an intermediate structure, the second step involves the sequestration of histories H2A/H2B onto DNA. Histones H2A/H2B are preferentially sequestered onto replicated DNA in comparison to non-replicated DNA incubated in the extract.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. T. Annunziato Split Decision: What Happens to Nucleosomes during DNA Replication? J. Biol. Chem., April 1, 2005; 280(13): 12065 - 12068. [Full Text] [PDF]
|
|
|
|
|
|
A. Tomita, D. R. Buchholz, K. Obata, and Y.-B. Shi Fusion Protein of Retinoic Acid Receptor {alpha} with Promyelocytic Leukemia Protein or Promyelocytic Leukemia Zinc Finger Protein Recruits N-CoR-TBLR1 Corepressor Complex to Repress Transcription in Vivo J. Biol. Chem., August 15, 2003; 278(33): 30788 - 30795. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-C. V. Hsia and Y.-B. Shi Chromatin Disruption and Histone Acetylation in Regulation of the Human Immunodeficiency Virus Type 1 Long Terminal Repeat by Thyroid Hormone Receptor Mol. Cell. Biol., June 15, 2002; 22(12): 4043 - 4052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Vestner, T. Waldmann, and C. Gruss Histone Octamer Dissociation Is Not Required for in Vitro Replication of Simian Virus 40 Minichromosomes J. Biol. Chem., March 10, 2000; 275(11): 8190 - 8195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Vestner, M. Bustin, and C. Gruss Stimulation of Replication Efficiency of a Chromatin Template by Chromosomal Protein HMG-17 J. Biol. Chem., April 17, 1998; 273(16): 9409 - 9414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Wong, Y B Shi, and A P Wolffe A role for nucleosome assembly in both silencing and activation of the Xenopus TR beta A gene by the thyroid hormone receptor. Genes & Dev., November 1, 1995; 9(21): 2696 - 2711. [Abstract] [PDF]
|
|
|
|
|
|
A. K. Eggleston, T. E. O'Neill, E. M. Bradbury, and S. C. Kowalczykowski Unwinding of Nucleosomal DNA by a DNA Helicase J. Biol. Chem., February 3, 1995; 270(5): 2024 - 2031. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M C Barton and B M Emerson Regulated expression of the beta-globin gene locus in synthetic nuclei. Genes & Dev., October 15, 1994; 8(20): 2453 - 2465. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Wolffe The role of transcription factors, chromatin structure and DNA replication in 5 S RNA gene regulation J. Cell Sci., January 8, 1994; 107(8): 2055 - 2063. [Abstract] [PDF]
|
|
|
|
|
|
G Almouzni and A P Wolffe Replication-coupled chromatin assembly is required for the repression of basal transcription in vivo. Genes & Dev., October 1, 1993; 7(10): 2033 - 2047. [Abstract] [PDF]
|
|
|
|
 |
|
 A.P. Wolffe, G. Almouzni, K. Ura, D. Pruss, and J.J. Hayes Transcription Factor Access to DNA in the Nucleosome Cold Spring Harb Symp Quant Biol, January 1, 1993; 58(0): 225 - 235. [Abstract] [PDF]
|
|
|
|
|
|
R. T. Richardson, I. N. Batova, E. E. Widgren, L.-X. Zheng, M. Whitfield, W. F. Marzluff, and M. G. O'Rand Characterization of the Histone H1-binding Protein, NASP, as a Cell Cycle-regulated Somatic Protein J. Biol. Chem., September 22, 2000; 275(39): 30378 - 30386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Ladoux, J.-P. Quivy, P. Doyle, O. d. Roure, G. Almouzni, and J.-L. Viovy Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy and scanning force microscopy PNAS, December 19, 2000; 97(26): 14251 - 14256. [Abstract] [Full Text] [PDF]
|
|