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Nucleic Acids Research, 1990, Vol. 18, No. 2 323-329
© 1990

MOLECULAR BIOLOGY

Characterizaton of the promoter region of Tetrahymena genes

Clifford F. Brunk and Lori A. Sadler

Biology Department and Molecular Biology Institute, University of California Los Angeles, CA 90024-1 606, USA

Received September 19, 1989. Revised December 11, 1989. Accepted December 11, 1989.

The regions between adjacent histone H3 and H4 genes, as well as portions of the genes, from 22 species of Tetrahymena have been amplified using the polymerase chain reaction and sequenced. Both histone genes are transcribed divergently with initiation occurring within the intergenic region, thus 2 sets of 22 homologous Tetrahymena promoters can be compared. A sequence comparison of these regions reveals a single putative promoter element, with a consensus sequence TATCCAATTCARA, present in front of each gene. This sequence contains a ‘CCAAT’ box, which also occurs at 8 locations preceeding other ciliate genes. No other putative promoter sequences are found in front of these sets of histone genes. Sequences searched for include ‘TATA’ boxes, ‘GC’ boxes and other sequences suggested as putative promoter elements for ciliate genes. The coding strand immediately preceeding ciliate genes is very high in A content and the consensus sequence at the site of protein synthesis is AAAATGG.


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