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Nucleic Acids Research, 1990, Vol. 18, No. 2 355-359
© 1990

MOLECULAR BIOLOGY

Cloning and nucleotide sequence of the gene encoding the Ecal DNA methyltransferase

Vladimir Brenner+, Pál Venetianer and Antal Kiss*

lnstitute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences PO Box 521, 6701 Szeged, Hungary

* To whom correspondence should be addressed

Received November 10, 1989. Accepted December 4, 1989.

The gene coding for the GGTNACC specific Ecal DNA methyltransferase (M.Ecal) has been cloned in E. coli from Enterobacter cloacae and its nucleotide sequence has been determined. The ecalM gene codes for a protein of 452 amino acids (Mr.: 51,111). It was determined that M.Ecal is an adenine methyltransferase. M.Ecal shows limited amino acid sequence similarity to other adenine methyltransferases. A clone that expresses Ecal methyltransferase at high level was constructed.

+Present address: Institute fo Microbiology, Department 112, Videnská 1083, Prague 4, 14200, Czechoslovakia


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